Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Yeast One-Hybrid (Y1H) System Service
The yeast one-hybrid system is a technique used to study the interaction between DNA and protein. Its characteristic is that it can identify proteins that are stably bound to DNA, can study the DNA-protein interactions in eukaryotes in yeast cells, and directly obtain the coding genes of target sequence interaction proteins by screening DNA libraries.
The principle of the yeast one-hybrid system is to construct a known specific cis-acting element structure upstream of the most basic promoter, and construct a reporter gene downstream of it. Then the fusion expression vector of the cDNA encoding the transcription factor to be tested and the known yeast transcription activation domain is introduced into yeast cells. If the gene can be combined with the cis-acting element, the most basic promoter can be activated and the reporter gene can be expressed.
Service introduction
Relying on the yeast hybridization technology platform, Creative BioMart provides one-stop services related to library construction, screening and verification of the yeast one-hybrid system to help researchers successfully achieve their research goals.
Yeast one-hybrid service projects we provide
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
The projects served are constantly updated, please contact us for more updated information.
Service items
Service content |
Customer needs to provide |
|---|---|
Library construction |
Cell / tissue / RNA sample |
Library screening |
1. Bait gene sequence / bait plasmid |
Point-to-point interaction verification |
1. pHis2-bait / pAbAi-bait plasmid |
Self-activation and toxicity verification |
1. pHis2-bait / pAbAi-bait plasmid |
Service process
Please let us know the details of your yeast hybridization related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
