Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Transcription Factor Self-Activation Verification Service
Transcription factor (TF), also known as trans-acting factor, refers to a DNA binding protein that can specifically interact with the cis-acting elements of eukaryotic genes and activate or inhibit gene transcription. TF plays an important regulatory role in the process of plant growth and development and adversity defense response. The functional study of TF and its interacting factors is essential to understand their role in the signal cascade, and provide theoretical basis for basic research and production applications.
The principle of transcription factor self-activation verification is as follows. According to the known regulatory proteins/transcription factors that can bind to the promoter, the bioinformatics method is used to predict the possible target regulatory proteins/transcription factors. After analysis, a reasonable truncation plan can be set to find core activation region of transcription factors conveniently and efficiently.
Service introduction
Creative BioMart combines many years of experience in yeast related research to provide self-activation verification service for transcription factors, which has great advantages in technology and quality.
Experimental steps for localization of transcription factor activation sequences
- Scheme design 1
- Scheme design 2
1. Smart database analysis
2. The first round of self-activation verification
- Vector construction of N-terminal and C-terminal
- Self-activation test of full-length, N-terminal and C-terminal
1. According to the results of the first round, design a truncation plan
2. The second round of self-activation verification
- Vector construction
- Self-activation verification test
Material requirements
- Templates and libraries
- Sequence
Delivery result
- Experiment report, including experimental equipment, reagents, methods, experimental steps, experimental results, experimental conclusions.
- Pictures
- Bacterial liquid or plasmids
Project cycle
It takes about 20 working days to complete transcription factor self-activation verification.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast related researches, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.