Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Yeast One-Hybrid (Y1H) Library Construction Service
Yeast one-hybrid is an effective method for cloning transcription factor genes (cDNA) that specifically bind to target elements based on the principle that DNA binding proteins (transcription factors) and DNA cis-acting elements are combined to regulate the expression of reporter genes.
The theoretical basis is that many eukaryotic transcription activators consist of physically and functionally independent DNA-binding domains (BD) and transcriptional activation domains (AD). Therefore, a fusion expression vector of various genes and AD can be constructed. When expressed as a fusion protein in yeast, a protein with a specific binding region to the target element can be screened according to the expression of the reporter gene. Theoretically, in one-hybrid detection, any target element can be used to screen for a protein with a specific binding region.
Service introduction
Recently, with the continuous development and improvement of the yeast one-hybrid system, its applications in scientific research and medical treatment have become more and more extensive. Creative BioMart combined years of library construction experience and launched a yeast one-hybrid library construction system. Thousands of libraries have been completed, which can effectively ensure the coverage of the library and the detection of the target gene for library screening.
Yeast one-hybrid library construction system we provide
- Clontech system
- Invitrogen system
Use SMART restriction enzyme digestion or in vivo recombination technology.
Use Gateway site-specific recombination technology.
| Step | Clontech system | Invitrogen system | |
|---|---|---|---|
| 1 | Total RNA | 1~2 μg | 350~500 μg |
| 2 | mRNA isolation | No need for mRNA isolation | Invitrogen patented kit |
| 3 | cDNA synthesis | SMART technology | Invitrogen patented high-efficiency kit |
| 4 | Library gene recombination | Restriction ligation or recombination in vivo | Gateway method for reorganization |
| 5 | Yeast transformation | Transform cDNA into yeast strain and recombine in vivo | The secondary library is directly transformed mixed plasmid into yeast strain |
Material requirements
- Cell sample > 1*107
- Animal sample > 1 g
- Plant samples > 2 g
- Total RNA > 500 μg
Delivery result
- Electronic report (library construction, yeast transformation)
- Primary library plasmid
- Bacteria from the secondary library
- Secondary library plasmid
- Yeast working solution
- All bacterial liquids and plasmids involved in library screening
Project cycle
If you don't need yeast transformation, we will deliver it within 30 working days. If needed, you need to extend 15 working days.
Service process
Please let us know the details of your yeast one hybridization related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.