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Yeast Library Screening Service for Transcription Factors

Transcription Factors (TFs) are proteins that can bind DNA in a sequence-specific manner and regulate transcription. Transcription factors control chromatin and transcription by recognizing specific DNA sequences to form a complex system that directs genome expression. Although many scientists have a keen interest in understanding how transcription factors control gene expression, it is still challenging to precisely locate the specific binding sites of transcription factors on the genome and how they ultimately participate in transcription regulation after binding. Creative BioMart has established a technology platform with transcription factor-centric technology to identify its recognition elements. This technology can accurately, quickly, simply and efficiently identify elements recognized by transcription factors, and has broad application prospects in the study of protein-DNA element interactions.

The experimental principle of the system is to introduce the method of sequence randomization to construct a random DNA library, and then use the yeast one-hybrid technology to screen the library to identify the DNA sequence that specifically binds to the transcription factor. The transcription factor studied is Bait, and a random short DNA sequence library is cloned into vector as Prey. One element sequence is enough to bind to TF.

Yeast Library Screening Service for Transcription Factors -Creative BioMart

Service introduction

Creative BioMart combines many years of experience in yeast related research to provide yeast library screening service for transcription factors, which has great advantages in technology and quality.

Material requirements

  • Templates and libraries
  • Sequence

Delivery result

  • Experiment report, including experimental equipment, reagents, methods, experimental steps, experimental results, experimental conclusions.
  • Bacterial liquid or plasmids

Project cycle

It takes about 20 working days to complete yeast library screening service for transcription factors.

Technical advantages

  • This technology can fully understand transcription factor recognition elements.
  • This technology can quickly identify various cis-acting elements bound by a specific transcription factor.
  • This technology expands the application range of yeast one-hybrid, making it not only for gene research, but also for transcription factor research.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Yeast Library Screening Service Process for Transcription Factors -Creative BioMart

Creative BioMart has accumulated many years of experience in the field of yeast related researches, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.