Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Prokaryotic Yeast One-Hybrid (Y1H) Service
Yeast one-hybrid technology is developed from the yeast two-hybrid technology. Through the phenotype detection of the reporter gene, the interaction between DNA and protein is analyzed to study the regulation of gene expression in eukaryotic cells. Due to the sensitivity and reliability of the yeast one-hybrid method to detect specific interactions between specific transcription factors and cis-acting elements, it has been widely used in cloned cells that contain weak specific transcription factors that are difficult to purify by biochemical means.
The theoretical basis of yeast one-hybrid (Y1H) is that the transcription activator of many eukaryotes is composed of DNA binding region (BD) and transcription activation region (AD), which are physically and functionally independent of each other. Therefore, a fusion expression vector of various genes and AD can be constructed. When expressed as a fusion protein in yeast, a protein with a specific binding region to the target element can be screened based on the expression of the reporter gene. Theoretically, in one-hybrid detection, any target element can be used to screen for a protein with a specific binding region.
Service introduction
Traditional yeast one-hybrid library construction methods are mostly for eukaryotes, because eukaryotic mRNA has a polyA structure. However, because bacterial prokaryotes do not have a polyA structure, it is difficult to construct a yeast one-hybrid library of bacterial prokaryotes using traditional methods. Creative BioMart has developed a special prokaryotic library construction and screening method for bacterial prokaryotes, which can stably, quickly and efficiently construct a high-quality yeast one-hybrid library of prokaryotic samples.
Experiment workflow
| Library construction process of prokaryotic yeast one-hybrid (Y1H) |
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| Library screening process of prokaryotic yeast one-hybrid (Y1H) |
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Material requirements
- Animal sample > 1g
- Plant sample > 2g
- Total RNA > 50μg
Delivery result
- Yeast one-hybrid library of glycerol bacteria (storage volume>1*107 CFU, average insert fragment > 1200bp, positive rate > 95%)
- Yeast one-hybrid library plasmid > 500μg
- Library construction report (electronic version)
- Library construction picture (JPG)
- Screening report, including sequencing results of positive clones
- Plasmid of positive clone
- Library screening picture (JPG)
Project cycle
A complete prokaryotic yeast one-hybrid (Y1H) service process takes about 50 working days.
Service process
Please let us know the details of your yeast one hybridization related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.