Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Yeast One-Hybrid (Y1H) Library Screening Service
Yeast one-hybrid technology was developed from yeast two-hybrid technology in 1993, and its basic principles are not complicated. The transcription initiation of eukaryotic genes requires the participation of transcription factors, which usually consist of a DNA specific binding domain (BD) and one or more activation domains (AD) interacting with other regulatory proteins. The yeast GAL4 protein used in the yeast one-hybrid system is a typical transcription factor. The DNA binding domain of GAL4 is close to the carboxyl end and contains several zinc finger structures, which can activate the upstream activation site (UAS) of yeast galactosidase, and the transcription activation domain can interact with RNA polymerase or transcription factor TFHID to increase the activity of RNA polymerase.
In this process, the DNA binding domain and transcription activation domain can function completely independently. Based on this, we can replace the DNA binding domain of GAL4 with library protein-encoding genes. As long as the expressed protein can interact with the target gene, RNA polymerase can also be activated through the transcription activation domain to initiate the transcription of downstream reporter genes.
Service introduction
Recently, with the continuous development and improvement of the yeast one-hybrid system, its applications in scientific research and medical treatment have become more and more extensive. Creative BioMart combined years of library screening experience and launched a yeast one-hybrid library screening system. Thousands of libraries have been completed, which can effectively ensure the coverage of the library and the detection of the target gene for library screening.
Experiment process
- cDNA library quality inspection
- Bait vector construction
- Bait plasmid self-activation test
- Co-transformation of yeast competent cells with library plasmids and bait plasmids
- Library screening
- Sequencing analysis of library screening results
- Organizing and sending detailed reports
Material requirements
- Information on bait plasmids used for screening
- Plasmids＞50 μg
Delivery result
- Detailed and complete screening experiment report
- Clear experiment pictures
- Sequencing results of screening results
- Plasmids for screening results
Project cycle
It takes about 25 working days to complete the complete library screening process of yeast one-hybrid.
Service process
Please let us know the details of your yeast one hybridization related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.