Genomic Yeast One-Hybrid (Y1H) Service

Yeast one-hybrid is an effective method for cloning transcription factor genes (cDNA) that specifically bind to target elements based on the principle that DNA binding proteins bind to DNA cis-acting elements to regulate the expression of reporter genes. The theoretical basis is that many eukaryotic transcription activators are composed of physically and functionally independent DNA-binding domains (BD) and transcriptional activation domains (AD). Therefore, various genes and AD can be constructed. When the fusion expression vector is expressed as a fusion protein in yeast, according to the expression of the reporter gene, the protein that has a specific binding region with the target element can be screened out. Theoretically, in one-hybrid detection, any target element can be used to screen for a protein with a specific binding region.

Service introduction

Creative BioMart provides yeast one-hybrid library construction and screening services for genomic samples. The length of the insert can be arbitrarily specified by you.

Experiment process

• Genome extraction
• Genome processing and intron removal (to ensure that the constructed library contains the complete coding region and does not contain intron sequences)
• Recombination of the processed genome with yeast one-hybrid library vector
• Electrotransformation of recombinant products
• Library detection and plasmid extraction

Genome yeast one-hybrid library screening

• Sequencing verification of bait vector and construction of one-hybrid screening vector
• Self-activation detection of decoy genes
• Transformation of bait plasmid into yeast cells
• Yeast screening
• Sequencing of screening results
• Rotation verification of screening results
• Submission of screening results, including screening reports and plasmids with positive results for screening.

Material requirements

• Cell sample > 1*10⁷
• Animal sample > 1 g
• Plant sample > 2 g
• DNA sample > 20 μg

Delivery result

• Yeast one-hybrid library of glycerol bacteria
• Yeast one-hybrid library plasmid > 500 μg
• Library construction report (electronic version)
• Library construction picture (JPG)
• (Optional) library glycerol bacteria transformed into yeast cells
• Library screening report, including sequencing results of screening results
• Plasmids for screening results

Project cycle

A complete genome yeast one-hybrid library construction service process takes about 50 working days.

Service process

Please let us know the details of your yeast one hybridization related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.