Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Yeast Library Construction Service for Transcription Factors
Transcription factors are the key components to achieve gene expression regulation and participate in every aspect of plant growth and development. In eukaryotes, about 5% of genes encode transcription factors, and about 50% are plant-specific transcription factors.
In the process of screening yeast single-hybrid cDNA libraries, customers often encounter unsuccessful screening of desired transcription factors. Because of this, Creative BioMart has compiled a database of transcription factors from multiple species and established a transcription factor-centric technology to identify its recognition elements. This technology can accurately, quickly, simply and efficiently identify the elements recognized by transcription factors, and has broad application prospects in the study of interactions between proteins and DNA elements.
The experimental principle of the system is to introduce the method of sequence randomization to construct a random DNA library, and then use the yeast one-hybrid technology to screen the library to identify the DNA sequence that specifically binds to the transcription factor. The transcription factor studied is Bait, and a random short DNA sequence library is cloned into the pHIS2 vector as Prey. One element sequence is enough to bind to TF.
Service introduction
Creative BioMart combines many years of experience in yeast related research to provide yeast library construction service for transcription factors, which has great advantages in technology and quality.
Material requirements
- Cell sample > 1*107
- Animal sample > 1 g
- Plant samples > 2 g
- Total RNA > 500 μg
Delivery result
- Experiment report, including experimental equipment, reagents, methods, experimental steps, experimental results, experimental conclusions.
- Bacterial liquid or plasmids
Project cycle
It takes about 20 working days to complete yeast library construction service for transcription factors.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.