Yeast Two-Hybrid (Y2H) System Service

The establishment of the yeast two-hybrid system (Y2H) is based on the understanding of eukaryotic regulation of transcription initiation. Initiation of gene transcription by cells requires the participation of trans-activator. The yeast two-hybrid system is to clone (fusion) the two proteins to be studied into the DNA binding domain (DNA-BD) and transcription activation domain (AD) of the transcription activator (such as modular GAL4, etc.) of the yeast expression plasmid. Above, it is constructed into a fusion expression vector, and the system for analyzing the interaction of two proteins from the expression product. When the two binding domains are separated, they still function separately, but they cannot activate transcription. When the two are relatively close in space through appropriate pathways, the GAL4 transcription factor activity can be reproduced, and the downstream promoter of the upstream activation sequence (UAS) can be activated, so that the downstream genes of the promoter can be transcribed.

Yeast Two-Hybrid (Y2H) Technology Principle -Creative BioMart

In the yeast two-hybrid system, the "bait" protein X is cloned into the DNA-BD vector to express the DNA-BD/X fusion protein; the protein Y to be tested is cloned into the AD vector to express the AD/Y fusion protein. Once there is an interaction between the X and Y proteins, DNA-BD and AD are also drawn closer, resume their functions, and activate the expression of LacZ and HIS3 genes in the reporter recombinant.

Service introduction

Relying on the yeast hybridization technology platform, Creative BioMart provides one-stop services related to library construction, screening and verification of the yeast two-hybrid system to help researchers achieve their research goals successfully.

Service items

Service item	Description
Yeast library construction	RNA extraction cDNA synthesis and linker connection cDNA is separated by length Recombination into yeast two-hybrid vector Library detection
Yeast library screening	Self-activation and toxicity biopsy Transformation of library plasmids Yeast hybridization and library screening Sequencing of screening results Rotation verification of screening results
Point-to-point verification	Construction Conversion Verification

Yeast two-hybrid service projects we provide

The projects served are constantly updated, please contact us for more updated information.

Service process

Please let us know the details of your yeast hybridization related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.