Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Yeast Two-Hybrid (Y2H) Library Screening Service
The protein yeast two-hybrid experiment is based on the genetic analysis of yeast, an experiment to study the influence of the interaction between trans-acting factors on the regulation of eukaryotic gene transcription. Transcription activating proteins can bind to specific sequences on DNA to initiate the transcription response of corresponding genes. This function of DNA binding and transcription activation is performed by two independent domains on transcription activation protein, namely DNA binding domain (BD) and transcription activation domain (AD). And these two domains are necessary for the transcriptional activation of genes.
The systems currently used in yeast two-hybrid experiments include LexA system and Gal4 system. In the LexA system, the DNA binding domain is composed of a complete prokaryotic protein LexA, and the transcription activation domain is composed of an acidic E. coli polypeptide B42 of 88 amino acids, which can activate gene transcription in yeast; In the Gal4 system, BD and AD are respectively composed of two different domains (1-147aa and 768-881aa) on the Gal4 protein. When using the GAL4 system to screen a cDNA library or study the interaction between proteins, the DNA binding domain binds to the target protein or "bait", and the transcription activation domain binds to the library protein or the protein to be verified.
Service introduction
Creative BioMart combines years of library construction experience to provide yeast two-hybrid (Y2H) library screening service.
Experiment process
- cDNA library quality inspection
- Bait vector construction
- Self-activation detection of bait plasmid
- Transformation of yeast competent cells with library plasmids and bait plasmids
- Library screening (mating or co-transfer)
- Sequencing analysis of library screening results
- Rotation verification of screening results (optional)
- Organize data and make detailed reports
- Delivery results
Delivery result
- Detailed screening report, including sequencing results of screening results.
- Library screening picture (JPG)
- Plasmids for screening results
- (Optional) Library glycerol bacteria
Project cycle
It takes about 45 working days to complete yeast two-hybrid library screening process.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.