High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service

Yeast two-hybrid (Y2H) technology is currently one of the most widely used methods in protein interaction research. The data obtained by using this technical method can help enrich the resource library of protein interactions, and can also help scientific researchers identify cancer subtypes and determine sub-networks related to plant pathogen infections. However, large-scale data collection and repeated screening in yeast two-hybrid are limited by cost and labor resources. The use of next-generation sequencing methods to identify protein interactions makes a large number of screenings feasible.

This technology advocates mixing all the bacteria together, and after high-throughput sequencing, agliment all the sequences using the bio-analysis method, which shortens the screening time and reduces the cost. And the larger the size of the sieve warehouse, the higher the efficiency, which makes the marginal cost drop rapidly. This enables large-scale and multiple repetitive screening and monitoring of protein interaction networks. In the era of big data, Creative BioMart has launched a yeast platform system + NGS technical service, through omics and bioinformatics analysis methods, comprehensively sorting out the vast omics information, and then using experimental verification methods, will quickly and effectively promote the yeast system research progress.

Service introduction

Creative BioMart combines years of library construction experience to provide high-throughput yeast two-hybrid (Y2H) library screening service.

High-throughput yeast two-hybrid (Y2H) library screening service

• High-throughput Y2H-seq

1. Bait library construction and prey library construction
2. Prey Library & Bait Library mating
3. Collection of positive clones and plasmid extraction
4. Electrotransfer to DH10B of the extracted plasmid library
5. Plasmid extraction and restriction digestion
6. Y2H-seq

• Bioinformatics analysis

7. Expression patterns of interacting proteins in different tissues
8. Cis-acting elements analysis of interacting proteins
9. GO function annotation of interacting protein

• Model prediction

10. Prediction of X-mediated regulation model

• Experimental verification

11. Y2H verification of interacting proteins

Material requirements

• Enough fresh samples
• Sequence information of bait and prey

Delivery result

• Raw data of second-generation sequencing
• Bioinformatics analysis report
• Original data, result picture
• Positive clone
• Positive clone plasmid
• Restriction digestion sample

Project cycle

It takes about 45 working days to complete high-throughput yeast two-hybrid (Y2H) library screening service.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.