Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
Yeast two-hybrid was based on the study of the properties of eukaryotic transcription factors (especially GAL4). GAL4 includes two domains, DNA binding domain (BD) and the transcription activation domain (AD). BD can recognize and bind to the upstream activation sequence (UAS) of GAL4-responsivegene. AD is through the combination with other components in the transcriptionmachinery to initiate transcription of genes downstream of UAS. BD and AD acting separately cannot activate the transcription reaction, but when the two are sufficiently close in space, they exhibit complete GAL4 transcription factor activity and can activate the promoter downstream of UAS, so that genes downstream of the promoter can be transcribed.
Fields has established a two-hybrid system in which BD is fused with X (X is often a known protein, BD-X is called bait), and AD is fused with Y (AD-Y is called prey). If a protein-protein complex is formed between X and Y, and the two domains of GAL4 are reconstituted, then the specific gene will be activated. Generally, reporter genes can show the interaction between bait and prey. Conversely, through the detection of the reporter gene, it can be judged whether there is an interaction between the bait and the prey.
Service introduction
Creative BioMart combines years of library construction experience to provide yeast two-hybrid (Y2H) verification service of nuclear system.
Experiment process
- Bioinformatics analysis of bait protein and prey protein
- Formulation of experimental protocol
- Construction of bait expression vector and prey expression vector
- Expression vector transformed yeast, verification and self-activation activity, toxicity test
- Co-conversion verification
- Make a display map
Delivery result
- Raw data
- Result picture
- Standard experiment report
Project cycle
It takes about 15 working days to complete yeast two-hybrid verification process of nuclear system.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.