Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
Based on the traditional yeast two-hybrid system, DUALmembrane technology cleverly uses the split-ubiquitin system to screen protein interactions. The split-ubiquitin system (split-ubiquitin) can be artificially divided into N-terminal (Nub) and C-terminal (Cub). Under normal circumstances, Nub and Cub still have a high affinity, showing the function of wild-type ubiquitin, that is, still playing the signal function of degrading proteins.
How to integrate the ubiquitin system with yeast two-hybrid? First, the 3rd isoleucine of ubiquitin Nub was artificially mutated to glycine (NubI was mutated to NubG). In this way, the affinity with Cub is greatly reduced, avoiding the possibility of self-association or proximity between Cub and Nub. Secondly, fusion Cub and synthetic transcription activator (LexA-VP16) into a fusion protein Cub-LexA-VP16. Therefore, under normal conditions, NubG does not bind to Cub, UAS cannot recognize isolated ubiquitin, and transcriptional activators will not be cut off. Finally, the protein to be detected is fused with NubG and Cub to form bait fusion protein (X-Cub-LexA-VP16) and prey fusion protein (Y-NubG). If bait and prey interact, it will prompt NubG and Cub to approach each other, leading to the dissociation of LexA-VP16, which will be recognized by UAS after entering the nucleus, thereby activating the transcription of the reporter gene.
Service introduction
Creative BioMart combines years of library construction experience to provide yeast two-hybrid (Y2H) library screening service of membrane system.
Experiment process
- Bioinformatics analysis of bait protein and prey protein
- Formulation of experimental protocol
- Construction of bait protein expression vector
- Bait function detection and bait vector selection
- 3-AT working concentration detection
- Library transformation and transfer of positive clones
- Sequencing analysis of library screening results
- Organize data and make detailed reports
Material requirements
- Decoy sequence
- Templates and libraries
Delivery result
- Detailed screening report, including sequencing results of screening results.
- Library screening picture (JPG)
- Plasmids for screening results
- (Optional) library glycerol bacteria
Project cycle
It takes about 45 working days to complete yeast two-hybrid library screening process of membrane system.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.