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Genomic Yeast Two-Hybrid (Y2H) Service

Genome Yeast Two-Hybrid (Y2H) ServiceThe yeast two-hybrid system is carried out in the eukaryotic model organism-yeast. It studies protein interactions in living cells, and the weak and instantaneous effects on proteins can also be sensitively detected by the expression products of reporter genes. It is a highly sensitive technique for studying the relationship between proteins. A large number of research documents have shown that the yeast two-hybrid technique can be used to study the interaction between the proteins encoded by the mammalian genomes, and it can also be used to study the interactions between the proteins encoded by the genomes of higher plants.

Service introduction

Creative BioMart provides yeast two-hybrid library construction and screening services for genomic samples. The length of the insert can be arbitrarily specified by you.

Experiment workflow

Genome yeast two-hybrid (Y2H) library construction process
  • Genome extraction
  • Genome processing and intron removal (to ensure that the constructed library contains the complete coding region and does not contain intron sequences)
  • Recombination of the processed genome with yeast two-hybrid library vector
  • Electrotransformation of recombinant products
  • Library detection and plasmid extraction
Genome yeast two-hybrid (Y2H) library screening
  • Sequencing verification of bait vector and construction of two-hybrid screening vector
  • Self-activation and toxicity detection
  • Transformation of bait plasmid into yeast cells
  • Yeast screening
  • Sequencing of screening results
  • Rotation verification of screening results
  • Submission of screening results, including screening reports and plasmids with positive results for screening.

Material requirements

  • Cell sample > 1*107
  • Animal sample > 1 g
  • Plant sample > 2 g
  • DNA sample > 20 μg

Delivery result

  • Yeast two-hybrid library of glycerol bacteria
  • Yeast two-hybrid library plasmid > 500 μg
  • Library construction report (electronic version)
  • Library construction picture (JPG)
  • (Optional) library glycerol bacteria transformed into yeast cells
  • Library screening report, including sequencing results of screening results
  • Plasmids for screening results

Project cycle

The project cycle is within 50 working days.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Genome Yeast Two-Hybrid (Y2H) Service Process -Creative BioMart

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.