Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
Protein-protein interactions are involved in most biological processes of cells, including gene expression, cell growth, proliferation, morphology, motility, and cell-to-cell communication. The use of the yeast two-hybrid (Y2H) system provides a definition of previously unknown pathways by characterizing novel interactions between proteins. This two-hybrid system relies on the bifunctional nature of transcription factors (such as yeast enhancer Gal4 or repressor LexA), so that protein-protein interactions can be monitored through the transcriptional changes of reporter genes.
After verifying the interactions between certain proteins, any kind of interacting proteins can be mutated through site-specific or randomly introduced changes, resulting in proteins with reduced interaction capabilities, and further study the relationship between its structure and function , To determine the key sites of interaction between proteins or individual amino acids and specific structures that play a decisive role. This type of technique is called the reverse two-hybrid (rY2H / rYTH) method.
Service introduction
Creative BioMart combines years of library construction experience to provide reverse yeast two-hybrid (rY2H) library construction service.
Technical methods of reverse two-hybrid library construction we provide
- Clonetech-SMART technology method
- Invitrogen-Gateway technical method
Use SMART restriction enzyme digestion or in vivo recombination technology.
Use Gateway site-specific recombination technology.
Reverse yeast two-hybrid (rY2H) library construction process
| Step | Clontech system | Invitrogen system | |
|---|---|---|---|
| 1 | Total RNA | 1~2 μg | 350~500 μg |
| 2 | mRNA isolation | No need for mRNA isolation | Invitrogen patented kit |
| 3 | cDNA synthesis | SMART technology | Invitrogen patented high-efficiency kit |
| 4 | Library gene recombination | Restriction ligation or recombination in vivo | Gateway method for reorganization |
| 5 | Yeast transformation | Transform cDNA into yeast strain and recombine in vivo | The secondary library is directly transformed mixed plasmid into yeast strain |
Material requirements
- Total RNA > 200 μg
- Animal sample > 1 g
- Plant sample > 2 g
- Cell sample > 1*107
Delivery result
- Reverse yeast two-hybrid library of glycerol bacteria
- Reverse yeast two-hybrid library plasmid
- Library construction report (electronic version)
- Library construction picture (JPG)
- (Optional) Library glycerol bacteria
Project cycle
It takes about 45 working days to complete reverse yeast two-hybrid library construction process.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.