Mammalian Two-Hybrid (mY2H) Service

With the rapid development of molecular biology research, especially the Human Genome Project, many new technologies have emerged in order to adapt to the development trend of functional research on many genes or proteins. Among them, the yeast two-hybrid technology has been widely used in the study of gene function because of its simplicity, sensitivity, high efficiency and ability to reflect the interaction between different proteins in living cells.

The protein yeast two-hybrid experiment is based on the genetic analysis of yeast to study the influence of the interaction between trans-acting factors on the regulation of eukaryotic gene transcription. Compared with previous experimental methods for studying protein-protein interactions, the yeast two-hybrid system has its unique advantages. 1. The interaction between the fusion protein is carried out in eukaryotic yeast cells, and the protein maintains its natural folded state, which is lacking in other biochemical testing methods in vitro. The interaction between the proteins confirmed by it will be closer The true level in the body. 2. The sensitivity of the two-hybrid system is very high, and it can be detected when the binding constant between proteins is as low as 1mmol / l. 3. When screening a cDNA library, the two-hybrid system can easily obtain the gene sequence encoding the interacting protein. It only needs to construct a plasmid without preparing antibodies or purifying proteins, and omits other in vitro methods for detecting interactions between proteins. Trivial steps such as protein extraction and purification.

Service introduction

Creative BioMart provides a yeast two-hybrid service for the nuclear/membrane protein system of mammalian cells, and you can choose a matching system for screening.

Yeast two-hybrid experiment procedure for mammalian cells

Material requirements

• Cell sample > 1*10⁷
• Animal sample > 1 g
• Plant sample > 2 g
• RNA sample > 200 μg
• For library screening, customers only need to provide the bait plasmid information for screening.

Delivery result

• Bacteria and plasmids of positive clones of yeast two-hybrid
• All original pictures related to the experiment
• Experiment report, including experimental equipment, reagents, methods, results, conclusions.

Project cycle

The experimental cycle of the standard process takes about 50 working days.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.