Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Yeast Three-Hybrid (Y3H) System Service
The yeast three-hybrid system is mainly used to study more complex macromolecular interactions including three components, providing a new method for the study of protein-protein, RNA-protein, and small molecule-protein interactions.
The three-hybrid system has a wide range of applications. 1. This system can be used to analyze and determine the precise domain of RNA-protein action, even a single nucleotide or amino acid residue. 2. It is used to identify and clone proteins that combine with important physiological functions of RNA (such as transcription, location, RNA virus packaging and infection, etc.), and can be used for regulatory mechanism research, disease prevention, and antiviral drug research and development. 3. By constructing a hybrid RNA library, RNA that binds to a specific protein can be screened. 4. On this basis, it is possible to develop a four-hybrid system to study the interaction between RNA-RNA.
Service introduction
Relying on the yeast hybridization technology platform, Creative BioMart provides one-stop services related to library construction, screening and verification of the yeast three-hybrid system to help researchers achieve their research goals successfully.
Service items
| Service item | Description |
|---|---|
| Yeast library construction | 1. RNA extraction and cDNA synthesis 2. cDNA ligation adapters, and fractionated according to length 3. Recombination of cDNA and yeast three-hybrid library vector 4. Transform the recombinant product into yeast cells |
| Yeast library screening | 1. Construction of bait vector 2. Self-activation detection of bait plasmid 3. Co-transformation of competent yeast cells with library plasmids and bait plasmids 4. Library screening and 3AT optimization 5. Sequencing analysis of library screening results 6. Screening results rotation verification |
Yeast three-hybrid service projects we provide
- Yeast Three-Hybrid (Y3H) Library Construction Service
- Yeast Three-Hybrid (Y3H) Library Screening Service
- Small Molecule Three-Hybrid (Y3H) Service for Drug Target Screening
The projects served are constantly updated, please contact us for more updated information.
Service process
Please let us know the details of your yeast hybridization related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
