Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Yeast Three-Hybrid (Y3H) Library Construction Service
The yeast three-hybrid (Y3H) system can be used for the experimental verification of the nucleic acid level in the cell. It has a wider range of applications than the yeast two-hybrid system. The third protein can make the two hybrid proteins close to each other, and is used to study the two proteins and the third. Interaction between proteins. The third component is different and can be protein, RNA or small molecules. At the same time, the mode of action of the third protein on the other two proteins (such as promotion or inhibition) can be studied through the growth status of the colony.
The basic principle of the yeast three-hybrid system is to construct the first fusion protein between a known RNA binding protein and the DNA binding domain (BD, such as LexA) of a transcription factor; Construct a fusion protein with the second protein (the RNA binding protein to be selected) and the transcription activation domain (AD); In addition, construct and express a hybrid RNA that contains two different binding sites. When RNA interacts with the binding sites of two RNA binding proteins, the transcription and expression of the reporter gene can be activated.
Service introduction
Creative BioMart combines many years of experience in yeast related research to provide yeast three-hybrid library construction service, which has great advantages in technology and quality.
Experiment process
1. RNA extraction and cDNA synthesis
2. cDNA ligation adapters, and fractionated according to length
3. Recombination of cDNA and yeast three-hybrid library vector
4. Transform the recombinant product into yeast cells
Material requirements
- Name and Gene ID of the protein to be studied
- Name and Gene ID of the RNA to be studied
- Cell sample > 1*107
- Animal sample > 1 g
- Plant samples > 2 g
- Total RNA > 500 μg
Delivery result
- Experiment report, including experimental equipment, reagents, methods, results, conclusions.
- Bacterial liquid and plasmids
Project cycle
It takes about 20 working days to complete yeast three-hybrid (Y3H) library construction service.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.