Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Yeast Surface Display Service
Yeast cell surface display technology is a new technology that is widely used to express proteins on the surface of yeast after translation and maturation in eukaryotic systems. The basic principle is to fuse a foreign target gene with a specific vector sequence and then introduce it into yeast cells, the fusion protein is induced to express, and the signal peptide guides the fusion protein to be secreted outside the cell. Since the fusion protein contains a structure that anchors the yeast cell wall, the fusion protein can be anchored in the yeast cell wall, so that the foreign protein molecule can be immobilized and expressed on the surface of the yeast cell.
Yeast is superior to phage in antibody display, because the eukaryotic expression environment of yeast cells is more suitable for protein folding, modification and translocation display. Therefore, the antibodies displayed by yeast are closer to their natural counterparts in conformation and function.
Yeast surface display is an effective protein engineering tool, which is widely used in protein evolution in vitro, antibody library screening, and antibody engineering in the fields of biotechnology and biomedicine. For example, it can be used to separate recombinant antibodies and T cell receptors that have binding specificity to a variety of proteins, peptides, and small molecules.
Service introduction
Creative BioMart R&D center has accumulated many years of technical experience to provide customers with mature and efficient yeast display services.
Types of Yeast Surface Display Services We Offer
- Yeast Surface Display Analysis of Agglutinin System
- Yeast Surface Display Analysis of Flocculin System
Service content
- Yeast display library construction
- Yeast display library screening
Experimental steps
- Recombination of DNA sequence with a display plasmid vector containing protein scaffold-Aga2 gene.
- This vector uses nutritional markers to maintain selective growth in yeast --- galactose is added to the medium to induce yeast for surface display.
- Combine magnetic beads and FACS sorting technology for multiple rounds of screening.
- Obtain high-affinity, high-stability clones.
Technical advantages
- Yeast has a secretion pathway similar to that of eukaryotes. Proteins are folded in the endoplasmic reticulum, where molecular chaperones, folding enzymes and quality control mechanisms ensure that only correctly folded proteins are secreted.
- The yeast display system uses FACS sorting technology, based on antibody affinity and display level, which can eliminate the deviation caused by expression and distinguish clones with low affinity.
- Yeast display is a kind of multivalent display, which can directly select clones with high, medium and low affinity through one sorting, which is very convenient.
- By using double-stained FACS, antibody affinity can be measured directly on the surface of yeast cells, so there is no need for time-consuming subcloning, expression and purification.
- In the same display system, the affinity and stability of antibodies can be optimized.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.