Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Yeast Surface Display Analysis of Agglutinin System
Yeast cell surface display technology is a eukaryotic protein expression system that uses a yeast cell surface display system (with a complete protein post-translational modification and secretion mechanism) to fix and display heterologous eukaryotic proteins on the surface of yeast cells. It has been widely used in many fields such as protein library screening, protein directed evolution, high-affinity antibody sorting, antigen/antibody library construction, affinity maturation, vaccine production, immune biocatalysis and biosensors.
The principle of the Agglutinin display and expression system is to connect the foreign gene with the C-terminal coding sequence of α-Agglutinin (containing the GPI anchor signal sequence) and insert it into the signal peptide downstream of the plasmid vector. After the fusion protein is induced to express, the signal peptide guides the secretion of the chimeric protein to the outside of the cell. Because there is an agglutinin polypeptide sequence containing a GPI anchor signal at the C-terminus of the fusion protein, the protein can be anchored in the yeast cell wall, so that the protein molecule can be displayed and expressed on the surface of the yeast cell.
Service introduction
Creative BioMart R&D center has accumulated many years of technical experience to provide customers with mature and efficient yeast surface display analysis of agglutinin system.
Type of agglutinin surface display system
- Yeast Surface Display Analysis of α-Agglutinin System (Agα)
- Yeast Surface Display Analysis of a-Agglutinin system (Aga)
Application of yeast cell surface display technology of agglutinin system
- Alcohol fermentation
α-agglutinin display system can display cellulolytic enzymes on the surface of Saccharomyces cerevisiae, and finally can directly convert amorphous cellulose into ethanol.
- New drug screening
- Biosorption
- Antigen surface display
- Other aspects
Display the gene products of unknown function or specific pathological process-related protein products on the surface of yeast, and then screen the cDNA expression library or mutant library. In this way, it is possible to discover substances that interact with these unknown function genes or related products of pathological processes, which is helpful for the study of unknown gene functions, or the screening of new drug lead compounds for the treatment of diseases.
Yeast cell surface display system can directly display heavy metals or certain organic chemical materials on the surface, and ultimately achieve the goal of bioremediation of environmental pollutants.
Displaying the antigen protein on the surface of yeast cells can make it closer to the natural spatial conformation, so that it can be subjected to serological testing such as ELISA.
Mutual recognition of protein molecules, directed evolution and immobilization of enzymes.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.