Yeast Surface Display Analysis of a-Agglutinin System (Aga)

The cell wall of Saccharomyces cerevisiae is mainly composed of the outer layer of mannoprotein and the inner layer of glucan skeleton, which are connected by covalent bonds. α-agglutinin and a-agglutinin are two mannoproteins on the cell wall of yeast. They mediate adhesion between the haploid cells of the α-mating type (MATα) and the α-mating type (MATa) of Saccharomyces cerevisiae, thereby making the cells fuse to form a diploid.

a-agglutinin is composed of core subunit Aga1 and binding subunit Aga2, which are connected by disulfide bonds. The C-terminus of the core subunit Aga1 is covalently bound to the dextran of the cell wall. The target protein is fused to the C-terminus of the binding subunit Aga2, that is, the N-terminus of the protein itself is fused to the C-terminus of the binding subunit Aga2 of a-agglutinin. Therefore, a reducing agent can be used to break the disulfide bond to dissociate the target protein from the cell surface, which is convenient for separation and purification.

Service introduction

Creative BioMart R&D center has accumulated many years of technical experience to provide customers with mature and efficient yeast surface display analysis of a-agglutinin system.

Features of yeast cell surface display technology

• The expression bias is small.
• The relative molecular mass range of fusion protein is wide.
• The number of fusion protein molecules is large.
• Convenient for screening, purification and activity determination.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.