Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Pichia pastoris Genome Editing Service
Yeast is a single-celled fungus, which has contributed to human society from ancient times to modern times. For thousands of years, in addition to its application in baking and fermentation, yeast has also become an important model organism in contemporary cell biology and microbiology research, such as Pichia pastoris. Because yeast is usually diploid or polyploid, its genetic manipulation is often difficult to complete. Mutagenesis breeding and genetic modification have been the mainstream methods of yeast genetic breeding for a long time. However, mutation breeding is not only a lot of work, but also has no clear directionality. Traditional genetic modification can only act on one resistance gene or modification site at a time. Greatly restricts the application of yeast in the microbial industry. With the increasing development of molecular biology research, there is an urgent need for a method that can quickly carry out genetic modification of yeast.
Red homologous recombination method and CRISPR-Cas9 system are widely used as simple, cost-effective and simple method for genome modification of model organisms. Yeast genome editing can help produce highly efficient mutant yeast colonies, thereby promoting its application in different industries such as biopharmaceuticals, pollution control, energy, and chemical and biological manufacturing.
Service introduction
Creative BioMart launched Pichia pastoris genome editing service based on red homologous recombination and CRISPR / Cas9. The mature Pichia pastoris editing system helps you successfully achieve gene knockout, gene insertion and point mutation.
Pichia pastoris genome editing technology
- Red homologous recombination method
- CRISPR/Cas9 method
Research content
- Pichia pastoris Gene Knockout Service
- Pichia pastoris Gene Knockin Service
- Pichia pastoris Gene Point Mutation Service
Service advantage
- Incognito editing
- Multi-gene editing --- it can knock out up to 3 genes at the same time.
- Easy to screen --- it does not require the target bacteria to be antibiotic resistant.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.