Pichia pastorise Gene Point Mutation Service

As a model strain of eukaryotes, Pichia pastorise is widely used in many fields such as medicine and food. Site-directed mutations of Pichia pastorise genes or chromosomal DNA sequences provide great reference for the study of eukaryotic gene functions.

A point mutation refers to a change in only one base pair. Point mutations in a broad sense can be base substitutions, single base insertions or base deletions. Narrowly sensed point mutations are also called single base substitutions. Base substitutions are divided into two types: transitions and transversions. Point mutation has a high rate of back mutation.

For site-directed mutations of the Pichia pastorise genome, Creative BioMart provides convenient and fast services to related researchers through improvements based on traditional homologous recombination methods and new CRISPR / Cas9 technology.

Service introduction

Creative BioMart researchers have established a mature yeast gene editing platform to provide high-quality gene point mutation service for Pichia pastorise and escort the success of your research.

Pichia pastorise gene point mutation technology

• CRISPR / Cas9 method

The CRISPR / Cas9 system is a natural defense system unique to bacteria and archaea, which is used to resist viruses or foreign plasmids.

• Homologous recombination method

1. Use the homologous recombination of Pichia pastorise to insert a selection marker at the position where the mutation is to be introduced.

2. Use the mutation-carrying DNA to knock out the introduced selection markers to achieve unit point mutations in the genome.

Customer provides information

• Pichia pastorise strain information
• ID or target sequence of the target gene
• Mutation site sequence information

Delivery result

• Experiment report, including experimental equipment, reagents, methods, results, conclusions.
• Edited strain

Project cycle

It takes about 35 working days to complete Pichia pastorise gene point mutation service.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.