Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Yeast WGBS Analysis
DNA methylation is an important epigenetic mark information and one of the means of gene regulation (that is, inhibiting gene expression by methylation of CpG islands located in the promoter and the first exon). It plays an important role in gene expression regulation, tumor research, chromatin remodeling, genetic imprinting, disease research, embryonic development, environment and epigenetics and epigenetic heterogeneity, etc., and is a hot spot in epigenetic research.
The experimental design idea of whole genome bisulfite sequencing (WGBS) is mainly difference comparison. The common type is the experimental group and the control group (case vs control), which is a pairwise comparison. Genomic DNA is treated with bisulfite to convert unmethylated cytosine (C) to uracil (U), while methylated C is unaltered. After PCR amplification, U will become T, which is distinguished from C, which is originally methylated. Then, by performing whole-genome resequencing on the processed DNA, single-base resolution and high-precision methylation analysis can be achieved from the genome level. WGBS has high precision and can obtain single-base precision biological methylation maps.
Service introduction
With an advanced yeast - centric technology platform, Creative BioMart offers its customers whole genome bisulfite sequencing (WGBS) services for yeast. We have strict quality control on every step of our service to ensure our clients receive high-quality data. If you have related needs, please contact us.
Technical route
Provided by clients
- Yeast cell number ≥ 5 × 107
- Yeast ≥ 200 mg (dry weight)
- DNA ≥ 10 μg, concentration ≥ 50 ng / μl
- Sample information sheet (the source, content, status and other basic information of the sample).
- It is recommended to set up 3 - 6 biological replicates to reduce within-group error and enhance the reliability of the results.
Delivery content
- Raw data
- Project analysis report, including experimental equipment, reagents, methods, results, conclusions.
Technical advantages of WGBS
- High measurement accuracy
It is single-base resolution for precise analysis of the methylation status of each C base.
- Wide detection range
It uses a high-throughput sequencing platform to study methylated regions on a genome-wide scale.
- High reliability
It directly sequences and quantifies methylated fragments without cross-reactivity and background noise.
- Cost-effective
Compared with the traditional PCR + Sanger sequencing method, it uses antibody - enriched hypermethylated region sequencing, which is less expensive.
Service process
Why choose us?
As a technology company focusing on yeast, Creative BioMart has sophisticated equipment, advanced technology and experienced personnel, and is committed to providing one-stop services for yeast-related services. If you would like to know more about our services, please feel free to contact us.