Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Yeast Surface Display Analysis of Glycosylphosphatidylinositol (GPI) Anchoring System
The flocculin display expression system utilizes the flocculin gene to fuse with foreign proteins and express the fusion protein on the cell surface. Its subsystem - GPI anchoring system uses the GPI signal contained in the C-terminus of the flocculin Flo1p to anchor foreign proteins. This system is similar to the agglutinin display system.
Yeast flocculation gene (Flo1) is a gene in the flocculation gene family of Saccharomyces cerevisiae that has been studied in detail, and the yeast surface display system is also operated based on this gene. Flo1 is dominant and encodes a lectin-like protein rich in serine and threonine. From N-terminus to C-terminus, Flo1 protein is composed of secretion signal region, 4-segment repeat sequence region and GPI anchor attachment signal region in sequence.
Service introduction
Creative BioMart R&D center has accumulated many years of technical experience to provide customers with mature and efficient yeast surface display analysis of glycosylphosphatidylinositol (GPI) anchoring system.
Technical advantages
- Yeast display is a kind of multivalent display, which can directly select clones with high, medium and low affinity through one sorting, which is very convenient.
- The yeast display system uses FACS sorting technology, based on antibody affinity and display level, which can eliminate the deviation caused by expression and distinguish clones with low affinity.
- By using double-stained FACS, antibody affinity can be measured directly on the surface of yeast cells, so there is no need for time-consuming subcloning, expression and purification.
- In the same display system, the affinity and stability of antibodies can be optimized.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.