Yeast Stress Resistance Gene Screening Service

Yeasts are eukaryotes, which are closer to plant and animal expression systems. They also have processes such as glycosylation, disulfide bond formation, and post-translational processing of protein folding, so that the proteins encoded by the selected plant genes can function normally, the probability of false positives for multiple adversity resistance genes is also greatly reduced.

Use yeast to detect whether the yeast expressing soluble protein can survive the stress under the stress system. Through the resistance gradient experiment, the resistance-related genes in the genome were screened, and the results of the screening were analyzed by bioinformatics, and these resistance genes were summarized and classified.

This technology is mainly used in the research of the molecular mechanism of drug resistance in medicinal organisms, the research of biological heavy metal repair mechanism, and the research of functional proteins for biological stress resistance.

Service introduction

Creative BioMart provides services for rapid screening of organisms' multiple stress resistance genes using yeast. According to gene homologous recombination, yeast mutants that are sensitive to various adversity conditions are screened. Thereby, the cDNA libraries of various animal and plant materials can be screened out for stress resistance genes that are resistant to multiple stresses, and their functional phenotypes can be verified.

Experimental steps

1. Adversity material

2. RNA extraction

3. Yeast expression library construction

4. Quality identification of yeast expression library

5. Yeast Working Bacteria Liquid

6. Adversity optimization

7. Yeast Stress Screening

8. Yeast positive clone identification

9. Yeast Induced Expression

10. Screening plate verification

11. Functional phenotype verification of stress resistance genes

Material requirements

• Name and Gene ID of the protein to be studied or plasmid
• Cell sample > 1*10⁷
• Animal sample > 1 g
• Plant samples > 2 g
• Total RNA > 500 μg

Delivery result

• Original picture
• Plasmid and bacterial solution
• Experiment report, including experimental equipment, reagents, methods, results, conclusions.

Project cycle

It takes about 20 working days to complete yeast stress resistance gene screening and verification service.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.