Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
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Yeast RNAi System
The Yeast RNAi system is a powerful tool for yeast rapid metabolic engineering. Creative BioMart's yeast RNAi system construction service provides a complete and customizable solution for researchers who need to study gene function or design yeast strains with the desired traits. Our services include RNAi construction design, cloning, transformation, screening, and validation, as well as necessary optimization. With our professional knowledge and cutting-edge technology, customers can confidently obtain high-quality and reliable results.
Our services can also be customized according to the specific needs and goals of each project. We can design and construct RNAi constructs that target single or multiple genes, and we can optimize expression conditions to achieve the best possible results. In addition, we regularly update project progress and work closely with clients to ensure their satisfaction and success.
Process of Yeast RNAi System Design Service
| Step | Description |
|---|---|
| Development of the experimental plan | We work closely with customers to understand their specific needs and goals for yeast metabolic engineering. We provide guidance and optimization for experimental design, and provide recommendations for specific metabolic pathways. |
| Design and construction of RNAi | We use cutting-edge bioinformatics tools to design RNAi constructs specifically targeting key genes in metabolic pathways of interest. Our RNAi constructs are designed for efficiency and specificity, ensuring maximum knockout of target genes. Then synthesize the RNAi construct and clone it into a suitable vector for expression in yeast. |
| Transforming the vector into yeast cells | Our expert team used a variety of methods to effectively transform RNAi constructs into yeast cells, including electroporation, chemical transformation and viral vector. We optimize the conversion method for each experiment based on the specific requirements of the customer. |
| Screening and validation | We used a combination of high-throughput analysis methods, including metabolomics and flowomics, to screen for metabolic flux changes in yeast strains produced. We also validated the knockout of target genes using qPCR and other gene expression analysis methods. |
| Optimization | We work closely with clients to optimize metabolic pathways of interest, utilizing the RNAi system to identify key regulatory genes and fine-tune metabolic flux. Our expert team provides guidance and support throughout the optimization process to ensure that customers achieve the desired results |
| Deliverable | We provide customers with the final RNAi-optimized yeast strains, as well as any relevant technical report and data analysis. |
Featured Service - Hairpin RNA (hpRNA) Expression Cassettes Construction
Figure 1. Schematic of a synthetic RNAi pathway in yeast. (Crook et al., 2013)hpRNA is one of the methods of RNAi, and we provide customers with design services for hpRNA expression cassettes in yeast. Our design includes the following steps:
- Selection of target genes.
- Design of hpRNA.
hpRNA is designed using specialized software that can predict the secondary structure and stability of stem ring structures. The length of the hairpin loop, connector sequence, and stem length will be optimized to achieve effective gene silencing.
- Selection of promoters.
Select the promoter used to drive hpRNA expression according to the specific requirements of the experiment. Typically, strong promoters are used, such as TEF1 or GPD1 promoters.
- Cloning of hpRNA expression cassette.
Synthesize hpRNA expression cassette and clone it into a suitable yeast plasmid vector. Then, standard transformation techniques are used to convert the plasmid into yeast cells.
- Verification of gene silencing.
Verify gene silencing by analyzing the expression level of target genes using techniques such as qPCR, Western blotting, and phenotype analysis.
Applications of Yeast RNAi System Design Service
- Research gene function and pathway analysis.
- Discovery of new drug targets and drug development.
- Production of recombinant proteins or metabolites.
- Improvement of industrial yeast strains for biofuels or pharmaceuticals.
Creative BioMart's yeast RNAi system services can benefit researchers who lack professional knowledge or resources to design, build, and optimize internal RNAi constructs. Our expert team has years of experience in RNAi technology, and we use the latest tools and technologies to ensure that our services have the highest level of effectiveness and reliability. Please feel free to contact us for more information.
Reference
- Crook, N. C., Schmitz, A. C., & Alper, H. S. (2013). Optimization of a yeast RNA interference system for controlling gene expression and enabling rapid metabolic engineering. ACS Synthetic Biology, 3(5), 307–313.