Yeast Protein Chip Service

Yeast is a single-celled eukaryotic organism. It is an important model organism in the fields of genetics, metabolism and gene function. It is also one of the first species to complete genome sequencing. The complete genome of Saccharomyces cerevisiae is 12068 kb, with 5885 open reading frames (ORFs) encoding proteins. In yeast and mammals, a large number of genes encode similar proteins. Among them, many genes involved in hereditary diseases have high homology. Studying the physiological functions of the proteins encoded by these genes and their interactions with other proteins will contribute to a better understanding of these inherited diseases. Zhu and other scientists obtained more than 5800 yeast proteins through recombinant expression and prepared them into protein chips, which provided a powerful tool for related scientific research.

Service introduction

With the advanced yeast center technology platform, Creative BioMart provides customers with yeast protein chip technology service. If you have any needs in this regard, please feel free to contact us.

Application fields

• The study of protein-protein interactions
• Research on protein-nucleic acid interactions
• Research on protein-small molecule interactions
• Research on post-translational modifications of proteins
• Biomarker discovery research
• Study of enzyme substrates

Specifications of yeast protein chips

Yeast protein	> 5800
The number of dots on a chip	1
Spotting environment	temperature 4 - 8 °C, humidity 30% ~ 40%
Chip substrate	75 * 25 mm
Dot diameter	~ 120 microns
Sample replicates	2 points / sample
Dot volume	0.5 ~ 1 nl / dot
Post - processing of chip spot production	Overnight fixation at 4 °C and long-term storage at - 80 °C

Features of yeast protein chips

• > 5800 yeast - encoded proteins cover more than 95% of the Saccharomyces cerevisiae proteome.
• Protein recombinant expression, with GST tag, and affinity purification.
• Set up two replicates on the chip for each protein.
• Protein expression preparation, chip preparation and storage are all carried out at low temperature.
• Fully ensure protein activity.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart's experienced technicians are well - positioned to optimize protocols to your requirements and ensure high-quality results. If you have specific related service needs, please contact us.

Reference

1. Zhu H, Bilgin M, Bangham R, et al. Global analysis of protein activities using proteome chips. Science, 2001, 293 (5537): 2101-2105.