Yeast Modified Qualitative Proteome Analysis Service

Conventional proteomic studies often only focus on changes in protein expression levels under different physiological and pathological conditions. However, more and more studies have found that many important life activities and disease occurrence are not only related to protein abundance, but also to protein modification. Therefore, in-depth study of protein modification is of great significance for revealing the mechanism of life activities, screening clinical markers of diseases and identifying drug targets.

The basic method of yeast modified proteome analysis is also relatively simple. Firstly, a yeast protein sample is digested into a peptide mixture. Secondly, liquid chromatography is used to separate the components of the peptide mixture to reduce sample complexity. Thirdly, the modified peptides are enriched by high-quality modified antibodies and biomaterials, and then loaded into liquid chromatography-tandem mass spectrometry for analysis. Finally, hundreds or thousands of modification sites can be identified at one time by searching for a match against the corresponding database.

Service introduction

Creative BioMart uses LC-MS/MS technology to provide identification services for modification sites analysis and expression profiling analysis for yeast proteins. If you have related needs, please contact us.

Types of yeast modified qualitative proteome analysis services

1. Yeast protein modification site analysis

Based on the characteristic that LC-MS/MS analysis can accurately determine the molecular mass of a protein or polypeptide, it can detect what kind of modification occurs on the protein or polypeptide. Detectable modification types are as follows: phosphorylation, glycosylation, acetylation, ubiquitination, propionylation, butyrylation, malonylation, glutarylation, succinylation, monomethylation, dimethylation, trimethylation.

Experiment workflow

Data analysis

• Identification of modified proteins
• Identification of modified peptides and sites

2. Yeast protein modification expression profiling

With the improvement of the sensitivity and resolution of mass spectrometry, the method of sample separation and classification has been further optimized, and the sequencing depth of PTM has been continuously increased. In terms of phosphorylation alone, deep sequencing technology has been able to determine more than 60,000 modification sites in humans, Arabidopsis and other organisms. The expression profiling studies on other PTMs are also constantly refreshing researchers' existing cognition. All this shows us the huge research potential of PTM expression profile.

Experiment workflow

Data analysis

• Protein identification
• Modification site identification
• Peptide conservation motif analysis
• GO function and KEGG signaling pathway analysis
• Protein interaction network analysis

Delivery content

• Raw data
• Project analysis report, including experimental equipment, reagents, methods, results, conclusions.

Project cycle

• The operating cycle of the standard process is 35 working days.

Service process

Creative BioMart has professional technology to provide customers with professional yeast-related research technical services. We can formulate the most suitable solution for your research according to different scientific research needs. If you want to know more service or technical details, please feel free to contact us.