Yeast MeRIP- seq Analysis

In recent years, scientists have discovered a reversible RNA methylation, that is, the methylation modification on the 6th nitrogen atom of adenine in the RNA molecule (N6-methyladenosine, m6A). m6A is a common post-transcriptional modification on eukaryotic mRNA and plays an important role in gene expression regulation, mRNA splicing, RNA editing, RNA stability, controlling mRNA lifespan and degradation, and mediating circular RNA translation.

At present, the most efficient method for m6A RNA detection is MeRIP-Seq, that is, Methylated RNA immunoprecipitation with next generation sequencing. This technique enriches hypermethylated RNA fragments using N6-methyladenine antibodies, followed by high-throughput sequencing. Studying the methylated RNA regions in the whole transcriptome, so as to compare the differences in RNA methylation modification between different samples, can help solve biological problems such as cell differentiation, biological development, disease occurrence and development, and heat shock response.

Service introduction

With an advanced yeast - centric technology platform, Creative BioMart offers MeRIP-seq services for m6A RNA methylation detection in yeast. We have strict quality control on every step of our service to ensure our clients receive high - quality data.

Experimental workflow

Provided by clients

• Yeast cell number ≥ 5 × 10⁷, yeast ≥ 200 mg (dry weight)
• RNA = 30 ~ 300 μg (OD 260 / 280 = 1.6 ~ 2.3, no RNA degradation, 28S / 18S > 1.5 or RIN > 7)
• Sample information sheet (the source, content, status and other basic information of the sample).
• Sample transportation

The sample is placed in a 1.5 mL centrifuge tube, sealed with parafilm, and shipped on dry ice.

• Sample storage

Yeast samples can be treated with TRIZOL or RNA protectant, and stored at -80°C after being frozen in liquid nitrogen.

RNA samples can be dissolved in ethanol or RNA - free ultrapure water and stored at -80°C to avoid repeated freezing and thawing.

Delivery content

Creative BioMart will provide you with detailed technical reports including:

• Experimental procedure
• Bioinformatics analysis results
• Display image of analysis results
• Raw data

Project cycle

• The operating cycle of the standard process is 45 working days.

Our advantages

• One-stop service

Customers only need to provide yeast samples, and we will complete the entire service process of enrichment - library preparation – sequencing - data analysis for you.

• Optimized experimental workflow

The enrichment efficiency of m6A RNA - seq is the key to determining the quality of the data, and we use validated commercial antibodies and carefully optimized experimental procedures with extremely high efficiency and specificity.

• Strict quality control

There are key quality controls throughout the experiment to ensure that customers get high-quality data.

• Professional bioinformatics analysis

We have a professional bioinformatics analysis team to help customers conduct comprehensive mining of experimental data.

• Combining RNA methylation sequencing with other omics data

We can integrate RNA methylation data and other omics data for bioinformatics association analysis to deeply mine the function of RNA methylation.

Service process

Why choose us?

With the advanced yeast center technology platform, Creative BioMart is committed to providing customers with a full range of yeast - related research service support and services. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.