Yeast Gene Knockout Service

The homologous recombination system is the most classic method of microbial gene editing, which can achieve various modifications such as knockout, point mutation, and knock-in of DNA molecules. This technology has been widely used in the research of genetic modification of genomic DNA, such as bacterial artificial chromosomes, E. coli chromosomes, and genetic engineering drugs.

Based on the classic homologous recombination system method, Creative BioMart has developed an innovative improved homologous recombination technology with knock-out efficiency and accuracy far higher than traditional methods after independent research and development and optimization of experimental procedures. This technique can be widely used in yeast gene editing.

Service introduction

Relying on years of rich experience in yeast-related research, Creative BioMart provides yeast gene knockout services. Based on traditional technology, improved to achieve higher accuracy and reorganization efficiency.

Experimental steps

1. Yeast resistance test

2. Construct recombinant vector

3. Transform recombinants into yeast competent cells

4. PCR identification and sequencing

5. Cultivate knock-out strains

Technical advantages

• Unique innovative technology to improve efficiency and accuracy
• Compared with traditional technology, gene knockout efficiency and recombination efficiency have been significantly improved
• Easily implement gene knockout, gene point mutation and gene knock-in

Material requirements

• Name and Gene ID of the protein to be studied
• Or plasmid

Delivery result

• Original picture
• Plasmid and bacterial solution
• Experiment report, including experimental equipment, reagents, methods, results, conclusions.

Project cycle

It takes about 20 working days to complete yeast gene knockout service.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.