Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Yeast Foreign Gene Secretion and Expression Service
Yeast has a protein secretion pathway similar to other eukaryotes. The secretion and expression of foreign genes in yeast not only facilitates the separation and purification of expression products, but also has important significance for studying the post-translational processing of expression products. Yeast expression system is a powerful tool for studying eukaryotic protein expression and analysis. Creative BioMart' yeast exogenous gene secretion expression system uses Pichia pastoris as the host. This expression system has developed rapidly in recent years and has been widely used. A kind of eukaryotic expression system.
Pichia pastoris is a methanol-trophic yeast with two alcohol oxidase (AOX) encoding genes AOX1 and AOX2. The sequences of these two genes are similar, and AOX1 is strictly induced and regulated by methanol. When methanol is the only carbon source, the AOX1 promoter can be induced by methanol to initiate the expression of ethanol oxidase. Thus, methanol is used for metabolism, and the plasmid containing the AOX1 promoter can be used to promote the expression of the target gene encoding the foreign protein.
Service introduction
Creative BioMart researchers have established a mature yeast expression and purification service platform to provide high-quality recombinant protein expression and purification services in Pichia pastoris.
Service content of yeast exocrine expression system
1. Construction of recombinant yeast expression plasmid
2. Electrotransformation of recombinant plasmids
3. Screening of recombinant expressors
4. Small-scale protein expression and purification
Material requirements
- Name and Gene ID of the protein to be studied
- Cell sample > 1*107
- Animal sample > 1 g
- Plant samples > 2 g
- Total RNA > 500 μg
Delivery result
- Gene clone (plasmid, Escherichia coli DH5α containing expression plasmid)
- Construction of Pichia pastoris expression vector (plasmid, positive expression strain)
- WB experiment results
- Original picture of protein purification
- Experiment report, including experimental equipment, reagents, methods, results, conclusions.
Project cycle
It takes about 25 working days to complete Pichia pastoris system protein (foreign gene) expression and purification service.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.