Yeast Display Service of IgG Full-length Antibody

Display technology is widely used in high-throughput screening of antibodies. At present, the commonly used phage display and yeast display technologies are mainly used to display single-chain variable region antibody fragments (ScFv). However, the antibody fragments screened by the display technology need to undergo structural conversion to convert the structure of the antibody fragments into the full-length IgG antibody structure for application in the development of antibody drugs. However, the full-length IgG antibody formed by structural conversion may lose its activity, and the converted full-length IgG antibody needs to be further matured and optimized in vitro to screen antibodies with high affinity. The most direct and effective strategy is to perform in vitro affinity maturation optimization screening by displaying IgG full-length antibodies.

Creative BioMart uses glycoengineered Pichia pastoris as a research tool to develop an IgG full-length antibody yeast display technology platform, which can replace phage and traditional yeast display technology to construct a large-capacity human IgG full-length antibody library for high-throughput affinity in vitro maturation screening, the selected high-affinity, high-expressing IgG full-length antibody does not require structural changes, and can be directly expressed for animal experiments and clinical experiments. It is a stable and efficient antibody affinity maturation system.

Service introduction

Creative BioMart provides yeast display service for IgG full-length antibodies, including one-stop service for antibody genetic engineering, including antibody humanization, antibody affinity maturation, and antibody expression and production.

Potential advantages of IgG full-length antibody yeast display technology

• Glyco-engineered Pichia pastoris has a quality control mechanism of eukaryotic expression system similar to animal cells, which can ensure the correct spatial structure folding of antibodies, and has a variety of post-translational processing functions such as human N-glycosylation, thus displaying IgG Antibodies are closest to natural antibodies in terms of molecular structure, physical and chemical properties and biological functions, and can be directly used in animal experiments and clinical trials.
• IgG full-length antibody yeast display technology has large antibody library capacity, simple operation, short cycle, low cost, and can screen IgG full-length antibodies with high throughput.
• The yeast display system uses FACS sorting technology to screen based on IgG antibody affinity and display level, which can eliminate deviations caused by different expression levels, and can distinguish clones with small differences in affinity.
• By using double-stained FACS, you can directly use yeast to display antibodies to determine antibody affinity without the need for time-consuming subcloning, expression and purification.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.