Yeast DIA Proteomic Analysis

DIA (Data Independent Acquisition) is a new mass spectrometry data acquisition method developed in recent years. It is an improvement on traditional shotgun analysis (in DDA mode, Data Dependent Analysis). It analyzes protein enzymatic peptide fragments by liquid mass spectrometry (LC-MS/MS), and combines the spectral library constructed by traditional DDA (Data Dependent Acquisition) to identify and quantify proteomes, thereby obtaining peptide fragments , protein and species source, protein expression changes and other information.

In DIA scan mode, all precursor ions in a specific mass range are first fragmented using ultra-high resolution mass spectrometry. Next, the fragment ions of all precursor ions are acquired, and all fragment ions within the adjacent precursor ion windows are quickly scanned in sequence. For example, taking 25 Da as a window, firstly separate peptides in the range of 500 - 525 Da, then collect peptides in the molecular weight range of 525 - 550 Da, and so on. Finally, the fragment ion information of all precursor ions was collected for protein identification and quantification.

Compared with DDA, DIA has better accuracy and repeatability.

Service introduction

Creative BioMart provides customers with DIA quantification services for yeast proteins. If you have any needs in this regard, please feel free to contact us.

Sample requirements

• Yeast ≥ 50 mg, or cell number ≥ 5×10⁷ 
• Sample information sheet (The source, content, status and other basic information of the sample should be filled in in detail).

Store in liquid nitrogen or -80°C.

Transport on sufficient dry ice to avoid repeated freezing and thawing.

Delivery content

• An experimental report, including specific experimental procedures and proteome identification and quantitative results (bioinformatics analysis).

Project cycle

• The operating cycle of the standard process is 45 working days.

Service process

Technical advantages

It is an improvement over traditional shotgun analysis (in DDA mode) that enables unbiased MS analysis of all signals in complex samples, resulting in higher peptide coverage. The DIA scanning mode divides the entire scanning range of the mass spectrometer into several windows, and selects, fragments, and detects all ions in each window at high speed and cyclically, so as to obtain all fragment information of all ions in the sample without omission and difference. information.

• High throughput

All target proteins can be monitored simultaneously.

• Increased number of identified proteins

Identification number increased by 30%.

• Good repeatability

Technical repeatability is increased by 30%, up to 90%.

• Good quantitative accuracy

Quality control CV is reduced by quantification of secondary peak area.

• No need to mark, preserve the authenticity of the sample to the greatest extent

Do you need more information? Please leave us a message via online inquiry. Creative BioMart is proud to serve clients from all over the world.

Reference

1. Bruderer R, Bernhardt O M, Gandhi T , et al. Extending the limits of quantitative proteome profiling with data-independent acquisition[C]// Proteomic Forum Berlin 2015. 2015.