Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Yeast CUT＆Tag Analysis
CUT&Tag (Cleavage Under Targets and Tagmentation) is a new technique for DNA-protein interaction studies that can be applied in the field of epigenetics, mainly for studying the binding or distribution sites of transcription factors or histone modifications on the whole genome, or for studying the interaction between proteins and DNA and identifying target DNA binding sites of target proteins. The technical objectives are similar to those of Chip-seq.
The core of the technology is hyperactive pG-Tn5 / pA-Tn5 transposase, which is a fusion of protein G/A with an engineered ultra-active Tn5 transposase, resulting in a novel fusion enzyme with both transposase and Protein G/A activity.
CUT & Tag works by first making the cell membrane permeable, then incubating the primary antibody with the target protein, then incubating with the secondary antibody, and finally adding the complex of proteinA-Tn5 and the sequencing junction. (by translocating the DNA around the target protein and ligating the sequencing junction). Finally, the sequencing library will be obtained by PCR.
Yeast needs to break the cell wall or extract the nucleus to perform the experiment.
Service introduction
Based on our powerful yeast technology center platform, Creative BioMart provides CUT & Tag analysis services for yeast epigenetic research. Combined with powerful biochemical analysis capabilities, Creative BioMart not only provides massive amounts of sequencing data, but can also mine and refine useful results and publication quality images targeted to your research goals. Please contact us if you have need.
Provided by clients
- Yeast cell number ≥ 5 × 107, yeast ≥ 200 mg (dry weight)
- Sample information sheet (the source, content, status and other basic information of the sample).
- Sample transportation
The sample is placed in a 1.5 mL centrifuge tube, sealed with parafilm, and shipped on dry ice.
Delivery content
Creative BioMart will provide you with detailed technical reports including:
- Experimental procedure
- Bioinformatics analysis results
- Display image of analysis results
- Raw data
Project cycle
- The operating cycle of the standard process is 45 working days.
Technical features
- Low cell starting volume
Compatible with as low as 60 cells (60-100,000 cells are sufficient), even single cells.
- Easy handling
No need for immunoprecipitation and ultrasonic fragmentation, no need for traditional end-repair, A-addition, and junctional library construction.
- Ultra-high activity
Combined pG/A & Tn5 activity, high DNA fragmentation activity
- High signal-to-noise ratio
- Good reproducibility
Service process
Why choose us?
With the advanced yeast center technology platform, Creative BioMart is committed to providing customers with yeast - related research service support and services. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.