Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Whole Transcriptome Sequencing and Analysis Service of Yeast
Yeast, as a widely used model organism, provides a convenient experimental system for higher eukaryotes, and is of great significance to food, medicine and industrial production. The current research on yeast-encoding genes is more in-depth and extensive, but its comprehensive three-dimensional regulation research is still in its infancy.
High-throughput sequencing of whole transcriptome, adopting ribosomal chain-specific library construction method and small fragment enrichment screening library construction method, which can realize the library construction, sequencing, information analysis and joint analysis of coding RNA and non-coding RNA, so as to quickly get comprehensive and accurate information about all RNA transcripts involved in specific biological processes (such as development, disease, etc.). This research extends the study of regulatory mechanisms to the three-dimensional model of the "line-surface-net" combination, which is helpful for a relatively comprehensive interpretation of biological phenomena.
Service introduction
Creative BioMart launched a yeast full-transcriptome high-throughput sequencing analysis service, which can identify lncRNA, small RNA, and circRNA, realize network analysis of the interaction of large RNA and small RNA, as well as endogenous competitive RNA identification and network analysis. If you have related needs, please contact us.
Sample requirements
- Total RNA ≥ 15 μg, concentration ≥ 100 ng / μl, RIN ≥ 7.5
Please put the sample in a 1.5 ml tube, indicate the name, concentration and preparation time of the sample on the tube, and seal the tube with parafilm. Before shipping, fix all sample tubes in 50 ml centrifuge tubes with caps and ship them on dry ice.
Sequencing strategy
- Two library sequencing: LncRNA-Seq + Small RNA
- Three library sequencing: LncRNA-Seq+ Small RNA+ CircRNA
The above two modes can also realize large RNA and small RNA interaction network analysis, as well as endogenous competitive RNA identification and network analysis.
| Research purposes | Library construction method | Sequencing mode | Clean data | Achieved results |
|---|---|---|---|---|
| LncRNA research | specific deribosomal chain construction | PE100 | 10G | Identification, quantification and functional analysis of lncRNA and mRNA. |
| Small RNA research | enrich small RNA fragments | SE50 | 20M | Identification, quantification and functional analysis of miRNA, siRNA and piRNA. |
| Circular RNA research | Remove linear RNA and reverse enrich circRNA | PE100 | 10G | Identification, quantification and functional analysis of low-abundance circular RNA. |
Analysis content
Project cycle
- The operating cycle of the standard process is 55 working days.
Delivery content
- Raw data
- Project analysis report, including experimental equipment, reagents, methods, results, conclusions.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.