Ubiquitination Proteomic Analysis of Yeast

Ubiquitination is an important post-translational modification. The ubiquitin-proteasome system mediates  80% to 85% of protein degradation in eukaryotes. In addition, ubiquitination can directly affect the activity and localization of proteins, and regulate various cellular activities including cell cycle, apoptosis, transcriptional regulation, DNA damage repair, and immune response. Owing to the low abundance and wide dynamic range of ubiquitination-modified proteins in biological samples. Therefore, before mass spectrometry analysis, it is necessary to enrich ubiquitinated peptide samples to increase the abundance of ubiquitinated proteins.

Creative BioMart uses mass spectrometry platforms (Q Exactive HF, Orbitrap Fusion, Orbitrap Fusion Lumos) combined with Nano - LC to launch a service technology package for ubiquitination quantitative proteome analysis of yeast. You only need to tell us the purpose of your experiment and send us your samples, we will take care of all the follow-up matters of the project, including protein extraction, protease digestion, ubiquitinated peptide enrichment, peptide separation, mass spectrometry analysis, raw Data analysis, bioinformatics analysis.

Service introduction

Creative BioMart provides a one-stop service for quantitative proteome analysis of yeast ubiquitination, if you have any needs in this regard, please feel free to contact us.

Provided by clients

• Yeast cell number ≥ 5 × 10⁷ 
• Yeast ≥ 200 mg (dry weight)
• Yeast protein extract ≥ 1 mg, concentration > 2 mg / ml.

Samples should not contain components that would interfere with separation, such as nucleic acids, lipids, and polysaccharides.

• Sample information sheet (the source, content, status and other basic information of the sample).

Experiment procedure

Delivery content

Creative BioMart will provide you with detailed technical reports including:

• Experimental procedure
• Relevant mass spectrometry parameters
• Details of the identified ubiquitination sites
• Mass spectrometry
• Raw data

Project cycle

• The operating cycle of the standard process is 45 working days.

Service process

Please let us know the details of your yeast hybridization related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart specializes in quantitative proteomics services for yeast post-translational modifications. Our researchers have many years of experience in this field. They can quickly understand the project situation through a simple description, and quickly formulate a feasible research plan according to customer needs. If you have this need, please feel free to contact us.