Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
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TMT Proteome Analysis of Yeast
TMT (tandem mass tags) is an in vitro tagging technology which is widely used in the analysis of differentially expressed proteins. This technology uses multiple isotopic tags to covalently bind to the amino group of the peptide, which can realize the simultaneous qualitative and quantitative analysis of proteins in different samples (currently, up to 16 samples can be simultaneously labeled).
Similar to iTRAQ, TMT reagents include 3 chemical groups in structure, namely reporter group, balance group and reactive group. The reactive group specifically reacts with the amino group of the peptide through covalent bonding, thereby connecting the TMT reagent to the peptide. In the primary spectrum, the same peptide from the same protein in different samples is shown as a peak because it is linked to the same total mass of intact TMT reagent. However, in the collision cell, the neutrality of the equilibrium group is lost, and the reporter group produces the corresponding reporter ions (6-plex: 126, 127, 128, 129, 130, 131 Da. 10-plex: 1126, 127N, 127C, 128N, 128C, 129N, 129C, 130N, 130C, 131 Da. 16-plex: 126, 127N, 127C, 128N, 128C, 129N, 129C, 130N, 130C, 131N, 131C, 132N, 132C, 133N, 133C, 134N). The intensities of these reporter ions represent the intensities of the corresponding sample proteins/peptides, enabling simultaneous quantification of 6 / 10 / 16 different samples.
Service introduction
Creative BioMart provides customers with TMT proteome quantification service for yeast, if you have any needs in this regard, please feel free to contact us.
Sample requirements
- Yeast ≥ 50 mg, or cell number ≥ 5×106
- Samples to be compared
- Sample information sheet (the source, content, status and other basic information of the sample should be filled in in detail).
Storage and transportation
- Store in liquid nitrogen or -80°C.
- Transport on sufficient dry ice to avoid repeated freezing and thawing.
Delivery content
- An experimental report, including specific experimental procedures and proteome identification and quantitative results (bioinformatics analysis).
Project cycle
- The operating cycle of the standard process is 35 working days.
Service process
TMT technical features
1. High throughput
It can analyze 6, 10, 16 samples at one time.
2. Good repeatability
The separation and identification conditions of all samples were the same.
3. Data rich
Qualitative and quantitative information is available for all proteins.
4. High degree of automation
Based on high-resolution LC/MS, automated operation and fast analysis speed.
5. Quantitatively accurate
It reduces the sample processing and the experimental error between different groups, so it can achieve a more accurate quantitative effect.
6. High sensitivity
Proteins with expression levels of picogram (pg) levels can be detected, and no high-abundance protein samples can be detected.
Why choose us?
Creative BioMart provides you with complete and optimized yeast-related analytical services. We can rely on the advanced yeast center technology platform and professional technicians to design solutions. We guarantee our clients the most reliable and efficient research services, as well as shorter turnaround times and lower prices. Do not hesitate to contact us.