Small RNA Sequencing and Analysis Service of Yeast

Yeast, as a widely used model organism, is of great significance to food, medicine and industrial production. At present, the research on yeast coding genes is more in-depth and extensive, but the research on smallRNA is still in its infancy.

Small RNA refers to endogenous non-coding RNA with a length of 18-30 nt, which is divided into three categories: microRNA (miRNA), small interfering RNA (siRNA) and RNA that interacts with piwi (piRNA). It is an important regulator of life activities. They control gene expression by silencing and degrading their target gene molecules. They play an important role in biological processes such as individual development, metabolism, and disease occurrence.

Next-generation sequencing technology provides a new and unique research platform suitable for deep identification and quantitative analysis of small RNA in yeast. Through in-depth sequencing, it is possible to discover, identify and quantify the small RNA map at the whole genome level of yeast, which advances the research on the function of small RNA.

Service introduction

Through the established yeast technology platform, Creative BioMart can perform deep sequencing and quantitative analysis of all small RNA in yeast cells. If you have related needs, please contact us.

Application area

• Studies on post-transcriptional regulation of yeast genes
• Yeast gene silencing research
• Yeast small RNA target analysis and regulatory network

Sample requirements

• Total RNA ≥10 μg, concentration ≥ 200 ng / μl (complete and non-contaminated RNA)
• Small RNA (< 200nt) ≥ 1 μg, concentration ≥ 20 ng / μl

Please put the sample in a 1.5 ml tube, indicate the name, concentration and preparation time of the sample on the tube, and seal the tube with parafilm. Before shipping, fix all sample tubes in 50 ml centrifuge tubes with caps and ship them on dry ice.

Experiment flow

Analysis content

• Standard information analysis

1. Data output statistics
2. The length distribution of small RNA
3. The distribution of small RNA on the selected reference genome
4. Small RNA classification statistics
5. miRNA quantitative analysis
6. miRNA differential expression analysis
7. miRNA expression/differential expression cluster analysis
8. miRNA target gene analysis
9. Differential miRNA target gene GO annotation and KEGG pathway analysis

• Advanced information analysis

1. Database notes

1) Transcription factor annotation analysis
2) GSEA analysis
3) Database notes for Rfam, Pfam, Reactome, COG, EggNOG and InterPro

2. Interaction network analysis

1) Target gene analysis
    miRNA-mRNA targeting relationship analysis
    LncRNA-mRNA targeting relationship analysis
2) ceRNA interaction network analysis
3) Protein interaction network analysis
4) Co-expression and interaction network analysis

Project cycle

• The operating cycle of the standard process is 45 working days.

Delivery content

• Raw data
• Project analysis report, including experimental equipment, reagents, methods, results, conclusions.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.