Shotgun Analysis Service for Yeast Protein

Shotgun analysis, also known as protein full-spectrum analysis, or protein components analysis, which can identify as many peptides and protein molecules as possible. The principle is to use shotgun technology to perform full-spectrum analysis of proteins. Firstly, a complex mixture of protein molecules in solution or SDS-PAGE bands is digested into a mixture of peptide fragments. Secondly, the peptides were separated by liquid chromatography (LC). Then, the peptides were identified using tandem mass spectrometry (MS/MS). This enables protein identification. Finally, the corresponding database is used to search and match, and hundreds of proteins can be identified at the same time.

Shotgun protein analysis can identify the entire protein of a sample, and can identify hundreds to thousands of proteins simultaneously. It is mainly applied to the identification of all expressed proteins in tissues, cells or organelles under a certain physiological state.

Service introduction

Based on ultra-high-resolution mass spectrometry technology, Creative BioMart provides shotgun analysis service of yeast proteins by combining mass spectrometry with chromatography, which can comprehensively identify a large number of proteins in whole yeast cell samples. If you have related needs, please contact us.

Sample requirements

• Sample type

Protein solution

• Sample size

Total protein > 10μg, solution concentration > 0.5μg /μl.

Buffer does not contain detergent NP40, Triton X-100, etc.

• Storage and shipping methods

Store at -80°C and ship on dry ice.

Delivery content

• Raw data
• Project analysis report, including experimental equipment, reagents, methods, results, conclusions.

Project cycle

• The operating cycle of the standard process is 35 working days.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. We strictly follow the experimental SOP for experimental optimization and analysis. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Why choose us?

Creative BioMart is a professional yeast-related research service provider, supporting professional yeast protein hybridization, protein expression, functional domain detection, omics research and other services. We can design and complete a range of solutions for service-themed projects. For more details on how we can support your project with innovation, please do not hesitate to contact us.