Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Saccharomyces cerevisiae Gene Point Mutation Service
As a model strain of eukaryotes, Saccharomyces cerevisiae is widely used in many fields such as medicine and food. Site-directed mutations of Saccharomyces cerevisiae genes or chromosomal DNA sequences provide great reference for the study of eukaryotic gene functions.
A point mutation refers to a change in only one base pair. Point mutations in a broad sense can be base substitutions, single base insertions or base deletions. Narrowly sensed point mutations are also called single base substitutions. Base substitutions are divided into two types: transitions and transversions. Point mutation has a high rate of back mutation.
For site-directed mutations of the Saccharomyces cerevisiae genome, Creative BioMart provides convenient and fast services to related researchers through improvements based on traditional homologous recombination methods and new CRISPR / Cas9 technology.
Service introduction
Creative BioMart researchers have established a mature yeast gene editing platform to provide high-quality gene point mutation service for Saccharomyces cerevisiae and escort the success of your research.
Saccharomyces cerevisiae gene point mutation technology
- CRISPR / Cas9 method
- Homologous recombination method
The CRISPR / Cas9 system is a natural defense system unique to bacteria and archaea, which is used to resist viruses or foreign plasmids.
1. Use the homologous recombination of Saccharomyces cerevisiae to insert a selection marker at the position where the mutation is to be introduced.
2. Use the mutation-carrying DNA to knock out the introduced selection markers to achieve unit point mutations in the genome.
Customer provides information
- Saccharomyces cerevisiae strain information
- ID or target sequence of the target gene
- Mutation site sequence information
Delivery result
- Experiment report, including experimental equipment, reagents, methods, results, conclusions.
- Edited strain
Project cycle
It takes about 35 working days to complete Saccharomyces cerevisiae gene point mutation service.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.