Saccharomyces cerevisiae Gene Knockout Service

Gene knockout is a foreign DNA introduction technology that uses a DNA fragment containing a certain known sequence to homologous recombination with a gene with the same or similar sequence in the genome of the recipient cell, and integrates it into the genome of the recipient cell and obtains expression. It is aimed at a sequence whose sequence is known but whose function is unknown. It changes the genetic gene of an organism, so that the function of a specific gene is lost, so that part of the function is blocked, and it can further affect the organism, and then infer the biological function of the gene.

Service introduction

Creative BioMart researchers have established a mature yeast gene editing platform to provide high-quality gene knockout services for Saccharomyces cerevisiae and escort the success of your research.

Saccharomyces cerevisiae genome knockout technology

• Red homologous recombination method

The Red system of Saccharomyces cerevisiae is used to carry out homologous recombination of foreign DNA to achieve allelic replacement of target genes. By designing the homologous fusion fragment of the target gene and cloning it into the suicide vector, the suicide vector is imported into the target bacteria by conjugation. Then, antibiotics are used to screen for the insertion mutant strains with the suicide vector integrated into the target site of the bacterial genome. Under the second round of reverse selection pressure, only the second homologous recombination of the Saccharomyces cerevisiae genome and the loss of the suicide plasmid can survive.

• CRISPR / Cas9 method

The CRISPR / Cas9 system is a natural defense system unique to bacteria and archaea, which is used to resist viruses or foreign plasmids. Creative BioMart launched the CRISPR method for Saccharomyces cerevisiae genome knockout services. Compared with the traditional gene knockout method, this method is fast and traceless, which is convenient for follow-up experimental research.

Customer provides information

• Saccharomyces cerevisiae host strain information
• ID or target sequence of the target gene

Delivery result

• Experiment report, including experimental equipment, reagents, methods, results, conclusions.
• Edited strain

Project cycle

It takes about 35 working days to complete Saccharomyces cerevisiae gene knockout service.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.