PRM-targeted Proteomic Analysis of Yeast

Parallel Reaction Monitoring (PRM) is an ion monitoring technology based on high-resolution and high-precision mass spectrometry, which can selectively detect target proteins and target peptides, so as to achieve accurate quantification of target proteins and peptides. Targeted proteome scanning is like a precision sniper, eliminating distractions and having a clear target.

Its technical principle is relatively simple. First, a quadrupole mass analyzer with high-resolution, high-precision mass spectrometry (such as the Orbitrap series) is used to selectively collect the target peptide precursorions in the primary mass spectrometry. Subsequently, the precursor ions are fragmented into fragment ions in the collision cell, and all enter the secondary mass spectrometer Orbitrap analyzer, which will produce a high-resolution secondary mass spectrum. Finally, all product ion information of the target peptide is collected to realize the specific analysis of target protein/peptide in complex samples.

Service introduction

Creative BioMart uses mass spectrometry platform (AB SCIEX Triple TOF 5600, Q Exactive, Fusion) combined with Nano - LC to launch PRM (targeted) quantitative proteome analysis service for yeast. You only need to provide us with the target protein information you need to study, and we provide one-stop PRM quantitative protein analysis services. If you have any needs in this regard, please feel free to contact us.

Experiment workflow

Provided by clients

• Yeast ≥ 50 mg, or cell number ≥ 5 × 10⁷ 
• Protein ≥ 100 μg, concentration ≥ 0.5 μg / μl
• Detected target protein and quantitative information in DDA mode (peptide segment of quantitative result, protein information table).
• Sample information sheet (The source, content, status and other basic information of the sample).

Delivery content

• An analysis report, including the experimental process, instrument model, parameters, reagent consumables, and quantitative result statistics.
• Quantitative result file (excel sheet)
• MS raw file. The optimized spectral library and the original data in wiff format collected by PRM were downloaded.

Project cycle

• The operating cycle of the standard process is 45 working days.

Service process

Technical features

• Accurate quantification.
• High throughput. 50 - 100 analytes can be monitored simultaneously.
• High sensitivity and high reproducibility.

Application scope

• After high-throughput quantitative proteomics, target protein validation was performed.
• Relative / absolute quantification of target proteins / peptides, qualitative quantification of protein post-translational modifications.

Do you need more information? Please leave us a message via online inquiry. Creative BioMart is proud to serve clients from all over the world.