MRM-targeted Proteomic Analysis of Yeast

MRM (Multiple Reaction Monitoring) is a targeted quantitative proteomics research method to study target protein molecules. Based on the information of target molecules, MRM selects data for mass spectrometry data acquisition. The signals that conform to the target ion rules are collected, and the interference of the ion signals that do not conform to the rules is removed.

MRM mass spectrometry analysis goes through three stages: 1. Precursor ions with specificity consistent with the target molecule are screened out by MS. 2. Collisional fragmentation of these precursor ions to remove interference from other ions. 3. Acquire the MS signal only for the selected specific MS / MS2 ions.

MRM mass spectrometry technology is a high-precision protein quantitative identification technology, and it is an excellent method for one-time accurate quantitative study of multiple target proteins in complex samples. If isotope-labeled target peptides are used as internal reference, absolute quantitative identification of proteins can be achieved.

Service introduction

Creative BioMart uses mass spectrometry platform (AB SCIEX TripleTOF 5600, Q Exactive, Fusion) combined with Nano-LC to launch MRM-targeted quantitative proteome analysis service for yeast. You only need to provide us with the target protein information you need to study, and we provide one-stop MRM quantitative protein analysis services. Including MRM method establishment and optimization, specific peptide selection, isotope labeling of peptides, mass spectrometry analysis, raw data analysis, bioinformatics analysis. If you have any needs in this regard, please feel free to contact us.

Experiment workflow

Sample requirements

• Yeast ≥ 50 mg, or cell number ≥ 5×10⁷ 
• Sample information sheet (The source, content, status and other basic information of the sample

Delivery content

• An experimental report, including specific experimental procedures and proteome identification and quantitative results (bioinformatics analysis).

Project cycle

• The operating cycle of the standard process is 45 working days.

Service process

Application areas of MRM technology

• Validation of non-targeted proteome research results such as Label-free
• Simultaneous absolute quantification of multiple proteins/peptides
• Study protein family changes with high homology but lack of specific recognition antibodies
• Quantitative studies of protein post-translational modifications

MRM technical features

• High sensitivity. After two-stage mass spectrometry, the ions consistent with the target ions are selected, the interference ions are eliminated, the signal-to-noise ratio is greatly improved, and the accuracy of the detection of the target ions is improved.
• High throughput, it can identify up to 200+ proteins at once.
• Absolute quantification can be performed without relying on antibodies.
• Identify low-abundance proteins with high accuracy, with quantitation spanning 4 orders of magnitude.

Do you need more information? Please leave us a message via online inquiry. Creative BioMart is proud to serve clients from all over the world.