Methylated Proteomic Analysis of Yeast

Protein methylation is one of the important research contents of epigenetics and one of the most common protein post-translational modifications. Methylation occurs mostly on transcription factors and nuclear proteins, but also on cytoplasmic proteins. Methylation can occur on arginine (R) and lysine (K), including monomethylation, asymmetric dimethylation / symmetric dimethylation, trimethylation and many other forms. Arginine methylation can regulate processes such as RNA processing, gene transcription, DNA damage repair, protein translocation and signal transduction. ysine methylation regulates histone function and participates in the epigenetic regulation of gene transcription. After enriching methylated peptides with specific antibodies against different methylation sites and modified forms, combined with LC - MS / MS analysis, large-scale quantitative and qualitative analysis of methylated proteins can be achieved. 

Service introduction

Creative BioMart uses Orbitrap Fusion Lumos mass spectrometry platform combined with nano LC - MS / MS chromatography to provide yeast methylation quantitative proteome analysis service technical services You only need to tell us the purpose of your experiment and send the sample, Creative BioMart provides one-stop services including protein extraction, protease digestion, methylated peptide enrichment, peptide separation, mass spectrometry analysis, mass spectrometry raw data analysis, and bioinformatics analysis. If you have any needs in this regard, please feel free to contact us.

Provided by clients

• Yeast ≥ 200 mg (dry weight), or cell number ≥ 5 × 10⁷ 
• Yeast protein extract ≥ 1 mg, concentration > 2 mg / ml. Samples should not contain components that would interfere with separation, such as nucleic acids, lipids, and polysaccharides.
• Sample information sheet (the source, content, status and other basic information of the sample).

Experiment procedure

Delivery content

Creative BioMart will provide you with detailed technical reports including:

• Experimental procedure
• Relevant mass spectrometry parameters
• Details of identified methylation sites
• Mass spectrometry
• Raw data

Project cycle

• The operating cycle of the standard process is 45 working days.

Service process

• You can tell us your needs through online inquiry or contact us directly
• Our experts quickly make a solution
• Sign contract and send samples
• One-stop service
• Project final report

Confidentiality statement

Creative BioMart has many business partners and understands the importance of confidentiality to these companies. We take confidentiality very seriously, sign a non-disclosure agreement before communicating with you, and employ multiple firewalls and safeguards to protect your privacy.

Creative BioMart's experienced technicians are well-positioned to optimize protocols to your requirements and ensure high-quality protein methylation analysis results. If you have specific related service needs, please contact us.