LncRNA Sequencing and Analysis Service of Yeast

Yeast, as a widely used model organism, is of great significance to food, medicine and industrial production. At present, the research on yeast coding genes is more in-depth and extensive, but the research on lncRNA is still in its infancy.

Long non-coding RNA (LncRNA) is a non-coding RNA with unique regulatory functions that has attracted widespread attention in recent years. The length of LncRNA is more than 200 nt, does not code for protein, and has poor conservation among species. Some sequence structures are similar to mRNA (containing polyA tails and variable splicing), while others do not have these characteristics. Studies have shown that lncRNA plays an important role in many life activities such as dosage compensation effect, epigenetic regulation, cell cycle regulation and cell differentiation regulation, and has become a research hotspot in genetics.

Service introduction

Creative BioMart uses high-throughput sequencing methods to screen LncRNAs in large quantities and quickly from the total RNA of yeast, as well as to annotate and predict their functions. If you have related needs, please contact us.

Sample requirements

• Total RNA ≥ 10 μg (concentration ≥ 300 ng/μl, OD 260 / 280 =1.8~2.2, OD 260 / 230 ≥ 2.0, RIN ≥ 7.0, 28S:18S ≥1.0)

Please put the sample in a 1.5 ml tube, indicate the name, concentration and preparation time of the sample on the tube, and seal the tube with parafilm. Before shipping, fix all sample tubes in 50 ml centrifuge tubes with caps and ship them on dry ice.

Experiment flow

Analysis content

• Standard Information Analysis

1) Sequencing data evaluation
2) Identification and annotation of known lncRNA
3) Transcript reconstruction and new lncRNA prediction
4) LncRNA expression statistics
5) LncRNA sample relationship analysis
6) LncRNA difference analysis
7) LncRNA family analysis

• Advanced Information Analysis

1) lncRNA location classification (Intergentic, Intronic, Cis-antisense)
2) Candidate lncRNA conservation analysis of
3) Correlation analysis (different levels of data of the same sample are required, such as correlation with sRNA sequencing results)
4) Candidate gene analysis
5) Customized chart
6) Customized personalized analysis

Project cycle

• The operating cycle of the standard process is 45 working days.

Delivery content

• Raw data
• Project analysis report, including experimental equipment, reagents, methods, results, conclusions.

Service process

Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.

Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.