iTRAQ Proteomic Analysis of Yeast

iTRAQ/TMT labeling quantitative proteome research is to label all proteins expressed in a genome or all proteins in a complex mixed system, and use the secondary reporter ions in the labeling reagent to accurately identify and quantify the proteins. There are two kinds of labeling reagents, namely iTRAQ (Isobaric Tag for Relative Absolute Quantitation) and TMT (Tandem Mass Tags), which are peptide labeling and quantitation technologies in vitro. They use tags of multiple isotopes, which can react with amino groups (including N-terminal amino acids and amino groups of lysine side chains) to achieve linkages. Through high-precision mass spectrometry analysis, they can simultaneously identify and quantify multiple sample proteomes.

• Reporter ion group

It used to quantify different peptides;

• Equilibrium group

It ensures the same quality of precursor ions after labeling the same peptide.

• Peptide reactive group

It reacts with amino acid N-terminal and lysine side chain amino group.

Service introduction

Creative BioMart provides customers with iTRAQ proteome quantification service for yeast, if you have any needs in this regard, please feel free to contact us. Our researchers have many years of experience in this field. They can quickly understand the project situation through a simple description, and quickly formulate a feasible research plan according to customer needs, saving time for the advancement of the project.

Sample requirements

• Yeast ≥ 50 mg, or cell number ≥ 5×10⁶ 
• Samples to be compared
• Sample information sheet (the source, content, status and other basic information of the sample should be filled in in detail).

Storage and transportation

• Store in liquid nitrogen or -80°C.
• Transport on sufficient dry ice to avoid repeated freezing and thawing.

Delivery content

• An experimental report, including specific experimental procedures and proteome identification and quantitative results (bioinformatics analysis).

Project cycle

• The operating cycle of the standard process is 35 working days.

Service process

Why choose us?

Creative BioMart provides you with complete and optimized yeast-related analytical services. We can rely on the advanced yeast center technology platform and professional technicians to design solutions. We guarantee our clients the most reliable and efficient research services, as well as shorter turnaround times and lower prices. Do not hesitate to contact us.