Glycosylation Proteomic Analysis of Yeast

Glycosylation is the process of linking sugar chains to proteins under the action of glycosyltransferases and forming glycosidic bonds with special amino acid residues on proteins. It is a common post-translational modification of proteins. It not only affects the spatial conformation, biological activity, transport and localization of proteins, but also plays a crucial role in specific biological processes such as molecular recognition, cellular communication, and signal transduction. 1. It has many functions such as protection, stability, organization and barrier to cells. 2. It can be used as a specific ligand for exogenous receptors, and some sugar chains can be used as specific receptors for various viruses, bacteria and parasites. 3. Sugar chains can also act as specific ligands for endogenous receptors and participate in mediating clearance, turnover and intracellular travel. 4. Sugar chains play an important role in the fertilization process.

With the continuous improvement of mass spectrometry resolution and the continuous development of bioinformatics, mass spectrometry has been widely used in glycosylation analysis. Usually, the glycan is released from the glycoprotein by enzymatic cleavage or chemical means, and then the identification of the glycosylation site and the analysis of the glycan structure are carried out respectively. Because glycosylation-modified proteins represent only a fraction of the total proteome, glycopeptide enrichment is often required.

Currently, there are five commonly used glycoprotein separation and enrichment techniques:

• Lectin affinity technique
• Hydrazine chemical enrichment method
• Boronic acid method
• Immunoassay
• Hydrophilic interaction chromatography (HILIC)

Service introduction

Creative BioMart uses mass spectrometry platforms (Q Exactive HF, Orbitrap Fusion, Orbitrap Fusion Lumos) combined with Nano - LC to launch a service technology package for glycosylation quantitative proteome analysis of yeast. You only need to tell us the purpose of your experiment and send us your samples, we will take care of all the follow-up matters of the project, including protein extraction, protease digestion, glycosylated peptide enrichment, peptide separation, mass spectrometry analysis, raw data analysis, bioinformatics analysis. if you have any needs in this regard, please feel free to contact us.

Provided by clients

• Yeast cell number ≥ 5 × 10⁷ 
• Yeast ≥ 200 mg (dry weight)
• Yeast protein extract ≥ 1 mg, concentration > 2 mg / ml.

Samples should not contain components that would interfere with separation, such as nucleic acids, lipids, and polysaccharides.

• Sample information sheet (The source, content, status and other basic information of the sample).

Experiment procedure

Delivery content

Creative BioMart will provide you with detailed technical reports including:

• Experimental procedure
• Relevant mass spectrometry parameters
• Details of the identified gycosylation sites
• Mass spectrometry
• Raw data

Project cycle

• The operating cycle of the standard process is 45 working days.

Service process

Creative BioMart specializes in quantitative proteomics services for yeast post-translational modifications. Our researchers have many years of experience in this field. They can quickly understand the project situation through a simple description, and quickly formulate a feasible research plan according to customer needs. If you have this need, please feel free to contact us.