Services
- Yeast Hybrid Services
- Yeast One-Hybrid (Y1H) System Service
- Yeast One-Hybrid (Y1H) Library Construction Service
- Yeast One-Hybrid (Y1H) Library Screening Service
- Prokaryotic Yeast One-Hybrid (Y1H) Service
- Genomic Yeast One-Hybrid (Y1H) Service
- Yeast One-Hybrid (Y1H) Point-to-Point Verification Service
- Yeast Library Construction Service for Transcription Factors
- Yeast Library Screening Service for Transcription Factors
- Transcription Factor Self-Activation Verification Service
- Yeast Two-Hybrid (Y2H) System Service
- Nuclear System Y2H Library Construction Service with Invitrogen Gateway Method
- Nuclear System Y2H Library Construction Service with Clontech-SMART Method
- Membrane System Y2H Library Construction Service with Invitrogen-Gateway Method
- Membrane System Y2H Library Construction Service with Clontech-SMART Method
- Reverse Yeast Two-Hybrid (rY2H) Library Construction Service
- Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Library Screening Service
- Membrane System Yeast Two-Hybrid (Y2H) Library Screening Service
- Nuclear System Yeast Two-Hybrid (Y2H) Verification Service
- Membrane System Yeast Two-Hybrid (Y2H) Verification Service
- High-throughput Yeast Two-Hybrid (Y2H) Library Screening Service
- Prokaryotic Yeast Two-Hybrid (Y2H) Service
- Genomic Yeast Two-Hybrid (Y2H) Service
- Mammalian Two-Hybrid (mY2H) Service
- Yeast One-to-One Verification Service
- Yeast Three-Hybrid (Y3H) System Service
- Yeast One-Hybrid (Y1H) System Service
- Yeast Expression System Services
- Yeast Foreign Gene Secretion and Expression Service
- Yeast Signal Peptide Tracking and Screening Service
- Yeast Stress Resistance Gene Screening Service
- Yeast Gene Knockout Service
- Yeast Surface Display Service
- Yeast Display Service of IgG Full-length Antibody
- Pichia pastoris Protein Expression Analysis
- Saccharomyces cerevisiae Protein Expression Analysis
- DNA Shuffling
- Yeast RNAi System
- Yeast Detection Services for Protein Functional Domain
- Yeast Microarray Chip Service
Online Inquiry
Genomics Sequencing and Analysis Service of Yeast
Yeast genome sequencing can be divided into yeast genome scanning, yeast genome fine map and yeast genome resequencing.
Yeast genome scanning is the study of obtaining a yeast genome framework map by sequencing yeasts with or without reference genomes through high-throughput sequencing technology. It can also carry out gene function annotation, comparative genomics and phylogenetic research based on the yeast genome framework map.
Yeast genome fine-grain sequencing, that is, de novo sequencing, means that a certain yeast species can be sequenced without any existing sequence information, and the sequence can be assembled by means of bioinformatics analysis. At present, second-generation sequencing combined with third-generation sequencing are commonly used to obtain fine-grained genome maps. Then, based on the fine map of the yeast genome, gene function annotation, comparative genomics and phylogenetic studies can be carried out.
Yeast genome resequencing is a high-throughput sequencing study based on yeast species with known reference genomes, generally focusing on genome variation (SNP, InDel, etc.), and group studies can also be performed. At the same time, it pays attention to a large number of single nucleotide polymorphism sites (SNP), insertion / deletion (InDel), structure variation (SV) and other variation information.
Service introduction
Creative BioMart can provide you with professional yeast genomics sequencing and analysis services according to your scientific research needs. If you have related needs, please contact us.
Technical method
- Yeast genome scanning
- Yeast genome fine map
- Yeast genome resequencing
Technology strategy
| Yeast genome scanning / resequencing | Yeast genome fine map | |
|---|---|---|
| Technology Strategy | lllumina PE150 (5Gb) | lllumina PE150 (5Gb) + third generation (100x) |
| Result characteristics | Get the genome framework map and carry out multi-database annotation and comparative genome research. | Scaffold N50>300k, more than 90% of contig and scaffold can be located on the chromosome. It can provide a variety of advanced analysis options and support the evaluation of personalized analysis methods to help in-depth scientific research. |
Analysis process
Sample requirements
- Total DNA ≥ 20 μg, concentration ≥20 ng/μl, OD 260 / 280 = 1.8 - 2.0.
- DNA is intact without degradation, and there is no contamination of RNA, protein, polysaccharides, etc.
Please put the sample in a 1.5 ml tube, indicate the name, concentration and preparation time of the sample on the tube, and seal the tube with parafilm. Before shipping, fix all sample tubes in 50 ml centrifuge tubes with caps and ship them on dry ice.
Delivery content
- Raw data
- Project analysis report, including experimental equipment, reagents, methods, results, conclusions.
Service process
Please let us know the details of your yeast related projects through online consultation. We will reply to you in time and provide you with a comprehensive evaluation report on your project, including the feasibility evaluation of the project and the detailed experimental plan designed by our professionals. As our condition optimization is completed, the result report and other final products will be delivered to you immediately.
Creative BioMart has accumulated many years of experience in the field of yeast research, helping our customers accelerate research in all fields using yeast as a research tool, and improve the overall success rate of the project. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.