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Genetic Engineering
Yeasts are a group of single-celled eukaryotes that reproduce asexually by budding or fission. They belong to the class of Ascomycetes, Basidiomycetes, and Deuteromycetes. They are composed of 56 genera and more than 500 species. If E. coli is the most mature prokaryotic expression system for foreign genes, then yeast is the most mature eukaryotic expression system.
Yeast is often used as a genetic engineering recipient, and it has the following advantages: 1. It has the characteristics of eukaryotes (protein post-translational processing and secretion system), clear genetic background, rapid growth, simple cultivation, complete foreign gene expression system, and genetic stability. 2. The endogenous protein products have a wide variety and high content. 3. It is suitable for the amplification and cloning of exogenous DNA, the high-efficiency expression of eukaryotic genes, the construction of gene libraries, and the study of eukaryotic gene expression regulation. It is an important eukaryotic receptor bacteria for DNA recombination experiments and genetic engineering. It is a genetically engineered receptor system recognized by the U.S. Food and Drug Administration as a safe (Generally Recognized As Safe GRAS).
Specific applications
- Modification of yeast itself to improve fermentation performance
- Using yeast as host to express heterologous proteins
- Selected recombinant proteins expressed by Saccharomyces cerevisiae
- Vaccine: Hepatitis B virus surface antigen, HIV-I capsid protein
- Diagnostic reagents: Hepatitis C virus protein HIV-I antigen
- Human therapeutic protein drugs: insulin-like insulin growth factor, platelet-derived growth factor, insulin precursor, fibroblast growth factor
- Industrial production
1. Introduce glucoamylase gene into Saccharomyces cerevisiae to facilitate the use of polysaccharides and dextrins, which account for 25% of the total wort sugars.
2. Introduce exogenous protein hydrolase gene into Saccharomyces cerevisiae to degrade the residual macromolecular proteins in the wort to avoid affecting the colloidal stability of beer.
3. Introduce 3-glucanase gene into yeast to degrade 3-glucan in wort to improve the filtration efficiency of beer.
4. Expression of ATP sulfatase and adenosine acyl sulfate kinase genes in Saccharomyces cerevisiae to obtain a yeast strain with greatly increased SO2 production, which promotes SO2 production and maintains the stability of beer flavor.
5. Transformation of the human serum albumin (HAS) gene into brewer's yeast to produce new HAS-rich beer varieties.
1. Expression level (mostly more than 1g/L)
human serum albumin, human lysozyme, Hansenula hepatitis B surface antigen, glucoamylase, endocannabinoidase
2. Expression quality
Industrial yeast strains for bioethanol and butanol production from C5 and C6 sugars
With the advanced yeast center technology platform, Creative BioMart is committed to providing customers with yeast - related research service support and services. Please tell us your project requirements, and we will provide you with a full service from strategy design to final report. If you have any questions, please feel free to contact us.